RAB5C Recombinant Rabbit Monoclonal Antibody [JE63-14]
cat.: HA721012
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE63-14 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 23 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human RAB5C aa 1-216/216. |
| Positive control: | 293 cell lysate, HepG2 cell lysate, LOVO cell lysate, SK-Br-3 cell lysate, human breast carcinoma tissue, human kidney tissue. |
| Subcellular location: | Early endosome membrane, Cell membrane, Melanosome. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:1,000 1:100-1:400 |
| Uniprot #: | SwissProt: P51148 Human |
| Alternative names: | L1880 RAB, member of RAS oncogene family like RAB5C RAB5C, member of RAS oncogene family RAB5C, member RAS oncogene family RAB5C_HUMAN RAB5CL RAB5L RABL Ras-related protein Rab-5C |
Images
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Fig1:
Western blot analysis of RAB5C on different lysates with Rabbit anti-RAB5C antibody (HA721012) at 1/500 dilution. Lane 1: 293 cell lysate Lane 1: HepG2 cell lysate Lane 1: LOVO cell lysate Lane 1: SK-Br-3 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 23 kDa Observed band size: 23 kDa Exposure time: 1 minute; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721012) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-RAB5C antibody (HA721012) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721012) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-RAB5C antibody (HA721012) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721012) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.