CD100 Recombinant Rabbit Monoclonal Antibody [JE63-21]
cat.: HA721013
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB
Clonality: Monoclonal
Clone number: JE63-21
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 96 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human CD100 aa 126-175/862.
Positive control: Jurkat cell lysate, Daudi cell lysate, HL-60 cell lysate.
Subcellular location: Cell membrane.
Recommended Dilutions:
  WB

1:500-1:2,000
Uniprot #: SwissProt: Q92854 Human
Alternative names: A8 BB 18 BB18 CD 100 CD100 CD100 antigen Coll 4 COLL4 Collapsin 4 Collapsin4 GR3 Leukocyte activation antigen CD100 M sema G MSEMA SEM4D_HUMAN Sema 4d Sema domain immunoglobulin domain Ig transmembrane domain TM and short cytoplasmic domain semaphorin 4D Sema H SEMA J Sema4d Semacl 2 Semacl2 SemaH SEMAJ Semaphorin 4D Semaphorin C like 2 Semaphorin H Semaphorin J Semaphorin-4D Semaphorin4D SemaphorinJ Semcl 2 Semcl2
Images
HA721013_1.jpg Fig1: Western blot analysis of CD100 on different lysates with Rabbit anti-CD100 antibody (HA721013) at 1/500 dilution.

Lane 1: Jurkat cell lysate
Lane 2: Daudi cell lysate
Lane 3: HL-60 cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 96 kDa
Observed band size: 130 kDa

Exposure time: 30 seconds;

8% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721013) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
HA721013_2.jpg Fig2: Western blot analysis of CD100 on different lysates with Rabbit anti-CD100 antibody (HA721013) at 1/2,000 dilution. Lane 1: HAP1-si NT cell lysate Lane 2: HAP1-si CD100 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 96 kDa Observed band size: 130 kDa Exposure time: 62 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721013) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.