CD100 Recombinant Rabbit Monoclonal Antibody [JE63-21]
cat.: HA721013
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE63-21 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 96 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human CD100 aa 126-175/862. |
| Positive control: | Jurkat cell lysate, Daudi cell lysate, HL-60 cell lysate. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB |
1:500-1:2,000 |
| Uniprot #: | SwissProt: Q92854 Human |
| Alternative names: | A8 BB 18 BB18 CD 100 CD100 CD100 antigen Coll 4 COLL4 Collapsin 4 Collapsin4 GR3 Leukocyte activation antigen CD100 M sema G MSEMA SEM4D_HUMAN Sema 4d Sema domain immunoglobulin domain Ig transmembrane domain TM and short cytoplasmic domain semaphorin 4D Sema H SEMA J Sema4d Semacl 2 Semacl2 SemaH SEMAJ Semaphorin 4D Semaphorin C like 2 Semaphorin H Semaphorin J Semaphorin-4D Semaphorin4D SemaphorinJ Semcl 2 Semcl2 |
Images
|
Fig1:
Western blot analysis of CD100 on different lysates with Rabbit anti-CD100 antibody (HA721013) at 1/2,000 dilution. Lane 1: HAP1-si NT cell lysate Lane 2: HAP1-si CD100 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 96 kDa Observed band size: 130 kDa Exposure time: 62 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721013) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of CD100 on different lysates with Rabbit anti-CD100 antibody (HA721013) at 1/1,000 dilution. Lane 1: Daudi (Human renal cell carcinoma cell) cell lysate Lane 2: Jurkat (Human T lymphocyte cell) cell lysate Lane 3: MOLT-4 (Human T lymphoblast cell) cell lysate Lane 4: THP-1 (Human leukemia monocytic cell) cell lysate Lane 5: HL-60 (Human promyelocytic leukemia cell) cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 46 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA721013, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 96.2 kDa Observed band size: 130 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.