GRSF1 Recombinant Rabbit Monoclonal Antibody [JE63-10]
cat.: HA721014
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P, FC
Clonality: Monoclonal
Clone number: JE63-10
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 53 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human GRSF1 aa 101-150/480.
Positive control: Hela cell lysate, 293 cell lysate, Raw264.7 cell lysate, mouse brain tissue lysate, mouse kidney tissue lysate, rat heart tissue lysate, human pancreas tissue, Daudi, Hela.
Subcellular location: Mitochondrion matrix; Cytoplasm.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC

1:500
1:50
1:400
1:500-1:1,000
Uniprot #: SwissProt: Q12849 Human | Q8C5Q4 Mouse
Entrez Gene: 305256 Rat
Alternative names: G rich RNA sequence binding factor 1 G rich sequence binding factor 1 G-rich sequence factor 1 GRSF-1 GRSF1 GRSF1_HUMAN
Images
HA721014_1.jpg Fig1: Western blot analysis of GRSF1 on different lysates with Rabbit anti-GRSF1 antibody (HA721014) at 1/500 dilution.

Lane 1: Hela cell lysate, 10 µg/Lane
Lane 2: 293 cell lysate, 10 µg/Lane
Lane 3: Raw264.7 cell lysate, 10 µg/Lane
Lane 4: Mouse brain tissue lysate, 20 µg/Lane
Lane 5: Mouse kidney tissue lysate, 20 µg/Lane
Lane 6: Rat heart tissue lysate, 20 µg/Lane

Predicted band size: 53 kDa
Observed band size: 53 kDa

Exposure time: 1 minute;

10% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721014) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
HA721014_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-GRSF1 antibody (HA721014) at 1/400 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721014) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721014_3.jpg Fig3: Flow cytometric analysis of Daudi cells labeling GRSF1.

Cells were fixed and permeabilized. Then stained with the primary antibody (HA721014, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
HA721014_4.jpg Fig4: Immunocytochemistry analysis of Hela cells labeling GRSF1 with Rabbit anti-GRSF1 antibody (HA721014) at 1/50 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-GRSF1 antibody (HA721014) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 647, HA1127) were used as the secondary antibody at 1/1,000 dilution.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.