GRSF1 Recombinant Rabbit Monoclonal Antibody [JE63-10]
cat.: HA721014
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JE63-10 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 53 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human GRSF1 aa 101-150/480. |
| Positive control: | Hela cell lysate, 293 cell lysate, Raw264.7 cell lysate, mouse brain tissue lysate, mouse kidney tissue lysate, rat heart tissue lysate, human pancreas tissue, Daudi, Hela. |
| Subcellular location: | Mitochondrion matrix; Cytoplasm. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500 1:50 1:400 1:500-1:1,000 |
| Uniprot #: | SwissProt: Q12849 Human | Q8C5Q4 Mouse Entrez Gene: 305256 Rat |
| Alternative names: | G rich RNA sequence binding factor 1 G rich sequence binding factor 1 G-rich sequence factor 1 GRSF-1 GRSF1 GRSF1_HUMAN |
Images
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Fig1:
Western blot analysis of GRSF1 on different lysates with Rabbit anti-GRSF1 antibody (HA721014) at 1/500 dilution. Lane 1: Hela cell lysate, 10 µg/Lane Lane 2: 293 cell lysate, 10 µg/Lane Lane 3: Raw264.7 cell lysate, 10 µg/Lane Lane 4: Mouse brain tissue lysate, 20 µg/Lane Lane 5: Mouse kidney tissue lysate, 20 µg/Lane Lane 6: Rat heart tissue lysate, 20 µg/Lane Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 1 minute; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721014) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-GRSF1 antibody (HA721014) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721014) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Flow cytometric analysis of Daudi cells labeling GRSF1. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721014, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig4:
Immunocytochemistry analysis of Hela cells labeling GRSF1 with Rabbit anti-GRSF1 antibody (HA721014) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-GRSF1 antibody (HA721014) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 647, HA1127) were used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.