CLPX Recombinant Rabbit Monoclonal Antibody [JE62-96]
cat.: HA721016
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE62-96 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 69 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human CLPX aa 584-633/633. |
| Positive control: | Raji cell lysate, Hela cell lysate, HepG2 cell lysate, NIH/3T3 cell lysate, rat heart tissue lysate, mouse heart tissue lysate, mouse liver tissue lysate, rat liver tissue lysate, rat testis tissue, mouse stomach tissue, NCI-H441. |
| Subcellular location: | Mitochondrion, mitochondrion nucleoid. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:500 1:50 1:100-1:400 |
| Uniprot #: | SwissProt: O76031 Human | Q9JHS4 Mouse | Q5U2U0 Rat |
| Alternative names: | ATP dependent Clp protease ATP binding subunit clpX like, mitochondrial ATP-dependent Clp protease ATP-binding subunit clpX-like, mitochondrial AU014732 Caseinolytic mitochondrial matrix peptidase chaperone subunit Caseinolytic peptidase X (E.coli) Caseinolytic protease X clpX ClpX caseinolytic peptidase X homolog ClpX caseinolytic protease X homolog CLPX_HUMAN E330029I21 Energy dependent regulator of proteolysis |
Images
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Fig1:
Western blot analysis of CLPX on different lysates with Rabbit anti-CLPX antibody (HA721016) at 1/500 dilution. Lane 1: Raji cell lysate Lane 2: Hela cell lysate Lane 3: HepG2 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 69 kDa Observed band size: 69 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721016) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of CLPX on different lysates with Rabbit anti-CLPX antibody (HA721016) at 1/500 dilution. Lane 1: NIH/3T3 cell lysate, 10 µg/Lane Lane 2: Rat heart tissue lysate, 20 µg/Lane Lane 3: Mouse heart tissue lysate, 20 µg/Lane Lane 4: Mouse liver tissue lysate, 20 µg/Lane Lane 5: Rat liver tissue lysate, 20 µg/Lane Predicted band size: 69 kDa Observed band size: 69 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721016) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-CLPX antibody (HA721016) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721016) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue with Rabbit anti-CLPX antibody (HA721016) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721016) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunocytochemistry analysis of NCI-H441 cells labeling CLPX with Rabbit anti-CLPX antibody (HA721016) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-CLPX antibody (HA721016) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 647, HA1127) were used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.