HINT1 Recombinant Rabbit Monoclonal Antibody [JE63-55]
cat.: HA721017
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE63-55 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 14 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human HINT1 aa 1-50/126. |
| Positive control: | Jurkat cell lysates, U87MG cell lysate, mouse liver tissue lysate, rat stomach tissue lysate, rat epididymis tissue, mouse stomach tissue. |
| Subcellular location: | Nucleus, Cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:1,000 1:400 |
| Uniprot #: | SwissProt: P49773 Human | P70349 Mouse | P62959 Rat |
| Alternative names: | Adenosine 5' monophosphoramidase Adenosine 5''-monophosphoramidase HINT 1 HINT1 HINT1_HUMAN Histidine triad nucleotide binding protein 1 Histidine triad nucleotide-binding protein 1 PKCI 1 PKCI-1 PKCI1 PRKCNH1 Protein kinase C inhibitor 1 Protein kinase C interacting protein 1 Protein kinase C-interacting protein 1 |
Images
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Fig1:
Western blot analysis of HINT1 on Jurkat cell lysates with Rabbit anti-HINT1 antibody (HA721017) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 14 kDa Observed band size: 13 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721017) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of HINT1 on different lysates with Rabbit anti-HINT1 antibody (HA721017) at 1/500 dilution. Lane 1: U87MG cell lysate, 10 µg/Lane Lane 2: Mouse liver tissue lysate, 20 µg/Lane Lane 3: Rat stomach tissue lysate, 20 µg/Lane Predicted band size: 14 kDa Observed band size: 13 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721017) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded rat epididymis tissue with Rabbit anti-HINT1 antibody (HA721017) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721017) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue with Rabbit anti-HINT1 antibody (HA721017) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721017) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.