| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | IHC-P, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | JE58-86 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 59 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human SLC45A3 aa 400-500. |
| Positive control: | Human prostate tissue, human prostate cancer tissue. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
IHC-P IF-Tissue |
1:200 1:200 |
| Uniprot #: | SwissProt: Q96JT2 Human |
| Alternative names: | IPCA 2 IPCA 8 IPCA6 PCANAP2 PCANAP6 PCANAP8 Prostate cancer associated gene 2 Prostate cancer associated gene 6 Prostate cancer associated gene 8 Prostate cancer associated protein 2 Prostate cancer associated protein 6 Prostate cancer associated protein 8 Prostate cancer-associated protein 6 Prostein PRST S45A3_HUMAN Slc45a3 Solute carrier family 45 member 3 |
|
Fig1: Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-SLC45A3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721018, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig2: Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-SLC45A3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721018, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Application: IF-Tissue Species: Human Site: prostate cancer Sample: Paraffin-embedded section Antibody concentration: 1/200 |