DENN Recombinant Rabbit Monoclonal Antibody [JE63-62]
cat.: HA721022
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE63-62 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 183 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human DENN aa 1,598-1,647/1,647. |
| Positive control: | HeLa cell lysate, Mouse brain tissue lysate. |
| Subcellular location: | Cell membrane, cytoplasm, axon. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: Q8WXG6 Human |
| Alternative names: | Differentially expressed in normal and neoplastic cells FLJ35600 IG20 Insulinoma glucagonoma clone 20 KIAA0358 MADD MADD_HUMAN MAP kinase-activating death domain protein Rab3 GDP/GTP exchange factor RAB3GEP DENN |
Images
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Fig1:
Western blot analysis of DENN on different lysates with Rabbit anti-DENN antibody (HA721022) at 1/1,000 dilution. Lane 1: HeLa cell lysate (negative) (20 µg/Lane) Lane 2: Mouse brain tissue lysate (40 µg/Lane) Predicted band size: 183 kDa Observed band size: 250 kDa Exposure time: 46 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721022) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.