DENN Recombinant Rabbit Monoclonal Antibody [JE63-62]
cat.: HA721022
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE63-62 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 183 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human DENN aa 1,598-1,647/1,647. |
| Positive control: | HepG2 cell lysate, Hela cell lysate. |
| Subcellular location: | Cell membrane, cytoplasm, axon. |
| Recommended Dilutions:
WB |
1:500 |
| Uniprot #: | SwissProt: Q8WXG6 Human |
| Alternative names: | Differentially expressed in normal and neoplastic cells FLJ35600 IG20 Insulinoma glucagonoma clone 20 KIAA0358 MADD MADD_HUMAN MAP kinase-activating death domain protein Rab3 GDP/GTP exchange factor RAB3GEP DENN |
Images
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Fig1:
Western blot analysis of DENN on different lysates with Rabbit anti-DENN antibody (HA721022) at 1/500 dilution. Lane 1: HepG2 cell lysate Lane 2: Hela cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 183 kDa Observed band size: 210 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721022) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.