Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human, Rat |
Applications: | WB |
Clonality: | Monoclonal |
Clone number: | JE63-65 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 156 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within human SARA aa 1-50/1,425. |
Positive control: | HUVEC cell lysate, rat brain tissue lysate, Hela cell lysate, SiHa cell lysate, PC-3 cell lysate. |
Subcellular location: | Early endosome membrane, Cytoplasm. |
Recommended Dilutions:
WB |
1:500-1,000 |
Uniprot #: | SwissProt: O95405 Human Entrez Gene: 313477 Rat |
Alternative names: | CG15667 hSARA LD33044p Madh-interacting protein MADHIP Mothers against decapentaplegic homolog-interacting protein Novel serine protease NSP Receptor activation anchor SAR1 SARA SARA1 Smad anchor for receptor activation SMADIP ZFYV9_HUMAN ZFYVE9 Zinc finger FYVE domain-containing protein 9 |
Fig1:
Western blot analysis of SARA on different lysates with Rabbit anti-SARA antibody (HA721023) at 1/500 dilution. Lane 1: HUVEC cell lysate, 10 µg/Lane Lane 2: Rat brain tissue lysate, 20 µg/Lane Predicted band size: 156 kDa Observed band size: 156 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721023) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of SARA on different lysates with Rabbit anti-SARA antibody (HA721023) at 1/1,000 dilution. Lane 1: Hela cell lysate Lane 2: SiHa cell lysate Lane 3: PC-3 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 156 kDa Observed band size: 156 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721023) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |