SARA Recombinant Rabbit Monoclonal Antibody [JE63-65]
cat.: HA721023
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE63-65 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 156 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human SARA aa 1-50/1,425. |
| Positive control: | HUVEC cell lysate, HeLa cell lysate, SiHa cell lysate, Jurkat cell lysate, MCF7 cell lysate, Rat brain tissue lysate. |
| Subcellular location: | Early endosome membrane, Cytoplasm. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: O95405 Human Entrez Gene: 313477 Rat |
| Alternative names: | CG15667 hSARA LD33044p Madh-interacting protein MADHIP Mothers against decapentaplegic homolog-interacting protein Novel serine protease NSP Receptor activation anchor SAR1 SARA SARA1 Smad anchor for receptor activation SMADIP ZFYV9_HUMAN ZFYVE9 Zinc finger FYVE domain-containing protein 9 |
Images
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Fig1:
Western blot analysis of SARA on different lysates with Rabbit anti-SARA antibody (HA721023) at 1/1,000 dilution. Lane 1: HUVEC cell lysate Lane 2: HeLa cell lysate Lane 3: SiHa cell lysate Lane 4: Jurkat cell lysate Lane 5: MCF7 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 156 kDa Observed band size: 156 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721023) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of SARA on different lysates with Rabbit anti-SARA antibody (HA721023) at 1/500 dilution. Lane 1: HUVEC cell lysate, 10 µg/Lane Lane 2: Rat brain tissue lysate, 20 µg/Lane Predicted band size: 156 kDa Observed band size: 156 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721023) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.