SARA Recombinant Rabbit Monoclonal Antibody [JE63-65]
cat.: HA721023
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Rat
Applications: WB
Clonality: Monoclonal
Clone number: JE63-65
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 156 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human SARA aa 1-50/1,425.
Positive control: HUVEC cell lysate, rat brain tissue lysate, Hela cell lysate, SiHa cell lysate, PC-3 cell lysate.
Subcellular location: Early endosome membrane, Cytoplasm.
Recommended Dilutions:
  WB

1:500-1,000
Uniprot #: SwissProt: O95405 Human
Entrez Gene: 313477 Rat
Alternative names: CG15667 hSARA LD33044p Madh-interacting protein MADHIP Mothers against decapentaplegic homolog-interacting protein Novel serine protease NSP Receptor activation anchor SAR1 SARA SARA1 Smad anchor for receptor activation SMADIP ZFYV9_HUMAN ZFYVE9 Zinc finger FYVE domain-containing protein 9
Images
HA721023_1.jpg Fig1: Western blot analysis of SARA on different lysates with Rabbit anti-SARA antibody (HA721023) at 1/500 dilution.

Lane 1: HUVEC cell lysate, 10 µg/Lane
Lane 2: Rat brain tissue lysate, 20 µg/Lane

Predicted band size: 156 kDa
Observed band size: 156 kDa

Exposure time: 2 minutes;

8% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721023) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
HA721023_2.jpg Fig2: Western blot analysis of SARA on different lysates with Rabbit anti-SARA antibody (HA721023) at 1/1,000 dilution.

Lane 1: Hela cell lysate
Lane 2: SiHa cell lysate
Lane 3: PC-3 cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 156 kDa
Observed band size: 156 kDa

Exposure time: 2 minutes;

10% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721023) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.