Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Mouse, Rat, Human |
Applications: | WB |
Clonality: | Monoclonal |
Clone number: | JE63-90 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 121 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within human LEKTI aa 151-200/1,064. |
Positive control: | Mouse lung tissue lysate, rat skin tissue lysate. |
Subcellular location: | Secreted. |
Recommended Dilutions:
WB |
1:500 |
Uniprot #: | SwissProt: Q9NQ38 Human Entrez Gene: 72432 Mouse | 361319 Rat |
Alternative names: | Hemofiltrate peptide HF7665 ISK5_HUMAN LEKTI Lympho-epithelial Kazal-type-related inhibitor Lymphoepithelial Kazal type related inhibitor NETS NS Serine peptidase inhibitor Kazal type 5 SPINK5 VAKTI |
Fig1:
Western blot analysis of LEKTI on different lysates with Rabbit anti-LEKTI antibody (HA721027) at 1/500 dilution. Lane 1: Mouse lung tissue lysate Lane 2: Rat skin tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 121 kDa Observed band size: 120 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721027) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |