LEKTI Recombinant Rabbit Monoclonal Antibody [JE63-90]
cat.: HA721027
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Mouse, Rat, Human
Applications: WB
Clonality: Monoclonal
Clone number: JE63-90
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 121 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human LEKTI aa 151-200/1,064.
Positive control: Mouse lung tissue lysate, rat skin tissue lysate.
Subcellular location: Secreted.
Recommended Dilutions:
  WB

1:500
Uniprot #: SwissProt: Q9NQ38 Human
Entrez Gene: 72432 Mouse | 361319 Rat
Alternative names: Hemofiltrate peptide HF7665 ISK5_HUMAN LEKTI Lympho-epithelial Kazal-type-related inhibitor Lymphoepithelial Kazal type related inhibitor NETS NS Serine peptidase inhibitor Kazal type 5 SPINK5 VAKTI
Images
HA721027_1.jpg Fig1: Western blot analysis of LEKTI on different lysates with Rabbit anti-LEKTI antibody (HA721027) at 1/500 dilution.

Lane 1: Mouse lung tissue lysate
Lane 2: Rat skin tissue lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 121 kDa
Observed band size: 120 kDa

Exposure time: 2 minutes;

6% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721027) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.