SPINK5 / LEKTI Recombinant Rabbit Monoclonal Antibody [JE63-90]
cat.: HA721027
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE63-90 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 121 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human LEKTI aa 151-200/1,064. |
| Positive control: | HeLa cell lysate, Mouse heart tissue lysate, Rat heart tissue lysate. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: Q9NQ38 Human Entrez Gene: 72432 Mouse | 361319 Rat |
| Alternative names: | Hemofiltrate peptide HF7665 ISK5_HUMAN LEKTI Lympho-epithelial Kazal-type-related inhibitor Lymphoepithelial Kazal type related inhibitor NETS NS Serine peptidase inhibitor Kazal type 5 SPINK5 VAKTI |
Images
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Fig1:
Western blot analysis of SPINK5 / LEKTI on different lysates with Rabbit anti-SPINK5 / LEKTI antibody (HA721027) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: Mouse heart tissue lysate (40 µg/Lane) Lane 3: Rat heart tissue lysate (40 µg/Lane) Predicted band size: 121 kDa Observed band size: 121 kDa Exposure time: 51 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721027) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.