| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE63-50 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 50 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human BLNK aa 357-456/456. |
| Positive control: | Raji cell lysate, Jurkat cell lysate, Mouse brain tissue lysate, Mouse spleen tissue lysate, Rat brain tissue lysate. |
| Subcellular location: | Cytoplasm, Cell membrane. |
| Recommended Dilutions:
WB |
1:2,000 |
| Uniprot #: | SwissProt: Q8WV28 Human | Q9QUN3 Mouse | Q4KM52 Rat |
| Alternative names: | AGM4 B cell adapter containing SH2 domain protein B cell adapter containing Src homology 2 domain protein B cell adaptor containing SH2 domain B cell linker B cell linker protein B cell-specific adaptor protein B-cell activation B-cell adapter containing a SH2 domain protein B-cell adapter containing a Src homology 2 domain protein B-cell linker protein BASH Bca BLNK BLNK s BLNK_HUMAN Cytoplasmic adapter protein Ly 57 Ly57 Lymphocyte antigen 57 Lyw 57 Lyw57 MGC111051 SH2 domain-containing leukocyte protein, 65-KD SLP 65 SLP-65 SLP65 Src homology [SH2] domain-containing leukocyte protein of 65 kD Src homology 2 domain containing leukocyte protein of 65 kDa Src homology 2 domain-containing leukocyte protein of 65 kDa |
|
Fig1:
Western blot analysis of BLNK on different lysates with Rabbit anti-BLNK antibody (HA721028) at 1/2,000 dilution. Lane 1: Raji cell lysate (20 µg/Lane) Lane 2: Jurkat cell lysate (20 µg/Lane) Lane 3: Mouse brain tissue lysate (40 µg/Lane) Lane 4: Mouse spleen tissue lysate (40 µg/Lane) Lane 5: Rat brain tissue lysate (40 µg/Lane) Predicted band size: 50 kDa Observed band size: 75 kDa Exposure time: 1 minute 7 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721028) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |