BLNK Recombinant Rabbit Monoclonal Antibody [JE63-50]
cat.: HA721028
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Mouse, Rat, Human
Applications: WB
Clonality: Monoclonal
Clone number: JE63-50
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 50 kDa
Isotype: IgG
Immunogen: Recombinant protein within human BLNK aa 357-456/456.
Positive control: Mouse spleen tissue lysate, rat brain tissue lysate.
Subcellular location: Cell membrane, Cytoplasm.
Recommended Dilutions:
  WB

1:500
Uniprot #: SwissProt: Q9QUN3 Mouse | Q4KM52 Rat | Q8WV28 Human
Alternative names: AGM4 B cell adapter containing SH2 domain protein B cell adapter containing Src homology 2 domain protein B cell adaptor containing SH2 domain B cell linker B cell linker protein B cell-specific adaptor protein B-cell activation B-cell adapter containing a SH2 domain protein B-cell adapter containing a Src homology 2 domain protein B-cell linker protein BASH Bca BLNK BLNK s BLNK_HUMAN Cytoplasmic adapter protein Ly 57 Ly57 Lymphocyte antigen 57 Lyw 57 Lyw57 MGC111051 SH2 domain-containing leukocyte protein, 65-KD SLP 65 SLP-65 SLP65 Src homology [SH2] domain-containing leukocyte protein of 65 kD Src homology 2 domain containing leukocyte protein of 65 kDa Src homology 2 domain-containing leukocyte protein of 65 kDa
Images
HA721028_1.jpg Fig1: Western blot analysis of BLNK on different lysates with Rabbit anti-BLNK antibody (HA721028) at 1/500 dilution.

Lane 1: Mouse spleen tissue lysate
Lane 2: Rat brain tissue lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 50 kDa
Observed band size: 70 kDa

Exposure time: 2 minutes;

10% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721028) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.