Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Mouse, Rat, Human |
Applications: | WB |
Clonality: | Monoclonal |
Clone number: | JE63-50 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 50 kDa |
Isotype: | IgG |
Immunogen: | Recombinant protein within human BLNK aa 357-456/456. |
Positive control: | Mouse spleen tissue lysate, rat brain tissue lysate. |
Subcellular location: | Cell membrane, Cytoplasm. |
Recommended Dilutions:
WB |
1:500 |
Uniprot #: | SwissProt: Q9QUN3 Mouse | Q4KM52 Rat | Q8WV28 Human |
Alternative names: | AGM4 B cell adapter containing SH2 domain protein B cell adapter containing Src homology 2 domain protein B cell adaptor containing SH2 domain B cell linker B cell linker protein B cell-specific adaptor protein B-cell activation B-cell adapter containing a SH2 domain protein B-cell adapter containing a Src homology 2 domain protein B-cell linker protein BASH Bca BLNK BLNK s BLNK_HUMAN Cytoplasmic adapter protein Ly 57 Ly57 Lymphocyte antigen 57 Lyw 57 Lyw57 MGC111051 SH2 domain-containing leukocyte protein, 65-KD SLP 65 SLP-65 SLP65 Src homology [SH2] domain-containing leukocyte protein of 65 kD Src homology 2 domain containing leukocyte protein of 65 kDa Src homology 2 domain-containing leukocyte protein of 65 kDa |
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Fig1:
Western blot analysis of BLNK on different lysates with Rabbit anti-BLNK antibody (HA721028) at 1/500 dilution. Lane 1: Mouse spleen tissue lysate Lane 2: Rat brain tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 50 kDa Observed band size: 70 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721028) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |