Cip4 Recombinant Rabbit Monoclonal Antibody [JE63-45]
cat.: HA721029
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE63-45 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 68 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human Cip4 aa 1-50/601. |
| Positive control: | JAR cell lysate, HepG2 cell lysate, 293T cell lysate, NIH/3T3 cell lysate, Mouse colon tissue lysate, PC-12 cell lysate, Rat brain tissue lysate. |
| Subcellular location: | Cell membrane, lysosome, cytoskeleton, golgi apparatus, cell cortex, phagocytic cup; perinuclear region. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: Q15642 Human | Q8CJ53 Mouse | P97531 Rat |
| Alternative names: | Cdc42 interacting protein 4 Cdc42 interaction protein 4 long isoform Cdc42-interacting protein 4 CG11341 CG15015 PA Cip 4 CIP4 CIP4_HUMAN DCIP4 hSTP Protein Felic Salt tolerant protein Salt tolerator STOT STP Thyroid hormone receptor interactor 10 Thyroid receptor interacting protein 10 Thyroid receptor-interacting protein 10 TR-interacting protein 10 TRIP 10 TRIP-10 trip10 Truncated Cdc42 interaction protein 4 |
Images
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Fig1:
Western blot analysis of Cip4 on different lysates with Rabbit anti-Cip4 antibody (HA721029) at 1/1,000 dilution. Lane 1: JAR cell lysate, 10 µg/Lane Lane 2: HepG2 cell lysate, 10 µg/Lane Lane 3: 293T cell lysate, 10 µg/Lane Lane 4: NIH/3T3 cell lysate, 10 µg/Lane Lane 5: Mouse colon tissue lysate, 20 µg/Lane Predicted band size: 68 kDa Observed band size: 80 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721029) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of Cip4 on different lysates with Rabbit anti-Cip4 antibody (HA721029) at 1/1,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-Cip4 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 68 kDa Observed band size: 80 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721029) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Western blot analysis of Cip4 on different lysates with Rabbit anti-Cip4 antibody (HA721029) at 1/1,000 dilution. Lane 1: PC-12 cell lysate, 10 µg/Lane Lane 2: Rat brain tissue lysate, 20 µg/Lane Predicted band size: 68 kDa Observed band size: 75 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721029) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.