HA721030

MEF2A + MEF2C Recombinant Rabbit Monoclonal Antibody [JE63-26]

cat.: HA721030

Product Type:	Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity:	Human, Mouse, Rat
Applications:	WB, IHC-P, FC
Clonality:	Monoclonal
Clone number:	JE63-26

Form:	Liquid
Storage condition:	Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer:	1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration:	1ug/ul
Purification:	Protein A affinity purified.
Molecular weight:	Predicted band size: 55/51 kDa
Isotype:	IgG
Immunogen:	Recombinant protein within human MEF2C aa 250-473/473.
Positive control:	Daudi cell lysates, rat skeletal muscle tissue, human striated muscle tissue, human glioma tissue, mouse hippocampus tissue, mouse brain tissue, Daudi.
Subcellular location:	Nucleus. Nucleus, sarcoplasm.
Recommended Dilutions: WB IHC-P FC	1:500 1:400 1:500-1:1,000
Uniprot #:	SwissProt: Q02078 Human \| Q06413 Human \| Q60929 Mouse \| Q8CFN5 Mouse \| Q2MJT0 Rat \| A0A096MJY4 Rat
Alternative names:	ADCAD1 MADS box transcription enhancer factor 2, polypeptide A (myocyte enhancer factor 2A) MEF2 MEF2A MEF2A_HUMAN Myocyte enhancer factor 2A Myocyte-specific enhancer factor 2A RSRFC4 RSRFC9 Serum response factor like protein 1 Serum response factor-like protein 1 C5DELq14.3 DEL5q14.3 MADS box transcription enhancer factor 2 polypeptide C (myocyte enhancer factor 2C) MADS box transcription enhancer factor 2, polypeptide C MEF2C MEF2C_HUMAN Myocyte enhancer factor 2C Myocyte specific enhancer factor 2C Myocyte-specific enhancer factor 2C OTTHUMP00000222409 Similar to MADS box transcription enhancer factor 2 polypeptide C

Images

	Fig1: Western blot analysis of MEF2A + MEF2C on Daudi cell lysates with Rabbit anti-MEF2A + MEF2C antibody (HA721030) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 55 kDa Observed band size: 55 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721030) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
	Fig2: Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue with Rabbit anti-MEF2A + MEF2C antibody (HA721030) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA721030) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig3: Immunohistochemical analysis of paraffin-embedded human striated muscle tissue with Rabbit anti-MEF2A + MEF2C antibody (HA721030) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA721030) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig4: Immunohistochemical analysis of paraffin-embedded human glioma tissue with Rabbit anti-MEF2A + MEF2C antibody (HA721030) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA721030) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig5: Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-MEF2A + MEF2C antibody (HA721030) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA721030) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-MEF2A + MEF2C antibody (HA721030) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA721030) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Fig7: Flow cytometric analysis of Daudi cells labeling MEF2A + MEF2C.

Cells were fixed and permeabilized. Then stained with the primary antibody (HA721030, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

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