FDPS Recombinant Rabbit Monoclonal Antibody [JE63-33]
cat.: HA721031
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, FC |
| Clonality: | Monoclonal |
| Clone number: | JE63-33 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 48 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human FDPS aa 370-419/419. |
| Positive control: | 293T cell lysate, HepG2 cell lysate, MCF-7 cell lysate, Hela cell lysate, Jurkat cell lysate, THP-1 cell lysate, THP-1. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB FC |
1:1,000 1:500-1:1,000 |
| Uniprot #: | SwissProt: P14324 Human |
| Alternative names: | (2E (2E,6E) farnesyl diphosphate synthase 6E)-farnesyl diphosphate synthase Dimethylallyltranstransferase Farnesyl diphosphate synthase Farnesyl diphosphate synthetase Farnesyl pyrophosphate synthase Farnesyl pyrophosphate synthetase Fdps FPP synthase FPP synthetase FPPS FPPS_HUMAN FPS Geranyltranstransferase |
Images
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Fig1:
Western blot analysis of FDPS on different lysates with Rabbit anti-FDPS antibody (HA721031) at 1/1,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-FDPS KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 48 kDa Observed band size: ~38 kDa Exposure time: 40 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721031) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of FDPS on different lysates with Rabbit anti-FDPS antibody (HA721031) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: HepG2 cell lysate Lane 3: MCF-7 cell lysate Lane 4: Hela cell lysate Lane 5: Jurkat cell lysate Lane 6: THP-1 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 48 kDa Observed band size: 39 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721031) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Flow cytometric analysis of THP-1 cells labeling FDPS. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721031, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.