BCKDK Recombinant Rabbit Monoclonal Antibody [JE63-77]
cat.: HA721032
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE63-77 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 46 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human BCKDK aa 50-100/412. |
| Positive control: | K562 cell lysate, Raji cell lysate, MCF-7 cell lysate, HUVEC cell lysate. |
| Subcellular location: | Mitochondrion matrix, Mitochondrion. |
| Recommended Dilutions:
WB |
1:500 |
| Uniprot #: | SwissProt: O14874 Human |
| Alternative names: | [3 methyl 2 oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial [3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase BCKD kinase BCKD-kinase BCKD_HUMAN BCKDHKIN Bckdk BCKDKD BDK Branched chain alpha keto acid dehydrogenase kinase Branched chain ketoacid dehydrogenase kinase Branched-chain alpha-ketoacid dehydrogenase kinase mitochondrial |
Images
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Fig1:
Western blot analysis of BCKDK on different lysates with Rabbit anti-BCKDK antibody (HA721032) at 1/500 dilution. Lane 1: K562 cell lysate Lane 2: Raji cell lysate Lane 3: MCF-7 cell lysate Lane 4: HUVEC cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 46 kDa Observed band size: 43 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721032) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.