BCKDK Recombinant Rabbit Monoclonal Antibody [JE63-77]
cat.: HA721032
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE63-77 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 46 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human BCKDK aa 50-100/412. |
| Positive control: | HAP1 cell lysate, K-562 cell lysate, Mouse kidney tissue lysate, Mouse pancreas tissue lysate, Rat pancreas tissue lysate. |
| Subcellular location: | Mitochondrion matrix. |
| Recommended Dilutions:
WB |
1:1,000-1:2,000 |
| Uniprot #: | SwissProt: O14874 Human |
| Alternative names: | [3 methyl 2 oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial [3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase BCKD kinase BCKD-kinase BCKD_HUMAN BCKDHKIN Bckdk BCKDKD BDK Branched chain alpha keto acid dehydrogenase kinase Branched chain ketoacid dehydrogenase kinase Branched-chain alpha-ketoacid dehydrogenase kinase mitochondrial |
Images
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Fig1:
Western blot analysis of BCKDK on different lysates with Rabbit anti-BCKDK antibody (HA721032) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-BCKDK KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 46 kDa Observed band size: 46 kDa Exposure time: 60 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721032) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of BCKDK on different lysates with Rabbit anti-BCKDK antibody (HA721032) at 1/2,000 dilution. Lane 1: K-562 cell lysate (20 µg/Lane) Lane 2: Mouse kidney tissue lysate (40 µg/Lane) Lane 3: Mouse pancreas tissue lysate (40 µg/Lane) Lane 4: Rat pancreas tissue lysate (40 µg/Lane) Predicted band size: 46 kDa Observed band size: 46 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721032) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.