HA721033

RSK2 Recombinant Rabbit Monoclonal Antibody [JE63-19]

cat.: HA721033

Product Type:	Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity:	Human, Mouse, Rat
Applications:	WB, IHC-P, FC
Clonality:	Monoclonal
Clone number:	JE63-19

Form:	Liquid
Storage condition:	Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer:	1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration:	1ug/ul
Purification:	Protein A affinity purified.
Molecular weight:	Predicted band size: 84 kDa
Isotype:	IgG
Immunogen:	Synthetic peptide within human RSK2 aa 661-710/740.
Positive control:	NIH/3T3 cell lysate, MCF-7 cell lysate, rat lung tissue lysates, human tonsils tissue, human liver carcinoma tissue, human colon carcinoma tissue, human skin tissue, human prostate tissue, human breast tissue, human breast carcinoma tissue, human pancreas tissue, mouse brain tissue, HepG2.
Subcellular location:	Nucleus, Cytoplasm.
Recommended Dilutions: WB IHC-P FC	1:500 1:200-1:400 1:500-1:1,000
Uniprot #:	SwissProt: P51812 Human \| P18654 Mouse Entrez Gene: 501560 Rat
Alternative names:	90 kDa ribosomal protein S6 kinase 3 CLS HU 3 HU2 HU3 Insulin stimulated protein kinase 1 Insulin-stimulated protein kinase 1 ISPK-1 ISPK1 KS6A3_HUMAN MAP kinase activated protein kinase 1b MAP kinase-activated protein kinase 1b MAPK activated protein kinase 1b MAPK-activated protein kinase 1b MAPKAP kinase 1b MAPKAPK 1b MAPKAPK-1b MAPKAPK1B Mental retardation, X linked 19 MRX19 OTTHUMP00000023036 p90 RSK2 p90 RSK3 p90-RSK 3 p90RSK3 pp90RSK2 Ribosomal protein S6 kinase 90kDa polypeptide 3 Ribosomal protein S6 kinase alpha 3 Ribosomal protein S6 kinase alpha-3 Ribosomal protein s6 kinase ii alpha 2 Ribosomal S6 kinase 2 Rps6ka3 RSK RSK-2 RSK2 S6 kinase 2 S6K alpha3 S6K-alpha-3

Images

Fig1: Western blot analysis of RSK2 on different lysates with Rabbit anti-RSK2 antibody (HA721033) at 1/500 dilution.

Lane 1: NIH/3T3 cell lysate
Lane 2: MCF-7 cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 84 kDa
Observed band size: 70 kDa

Exposure time: 2 minutes;

8% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721033) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

Fig2: Western blot analysis of RSK2 on rat lung tissue lysates with Rabbit anti-RSK2 antibody (HA721033) at 1/500 dilution.

Lysates/proteins at 20 µg/Lane.

Predicted band size: 84 kDa
Observed band size: 84 kDa

Exposure time: 2 minutes;

8% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721033) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

	Fig3: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue with Rabbit anti-RSK2 antibody (HA721033) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA721033) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig4: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-RSK2 antibody (HA721033) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA721033) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig5: Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-RSK2 antibody (HA721033) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA721033) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig6: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-RSK2 antibody (HA721033) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA721033) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig7: Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-RSK2 antibody (HA721033) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA721033) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig8: Flow cytometric analysis of HepG2 cells labeling RSK2. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721033, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.