RSK2 Recombinant Rabbit Monoclonal Antibody [JE63-19]
cat.: HA721033
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, FC
Clonality: Monoclonal
Clone number: JE63-19
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 84 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human RSK2 aa 661-710/740.
Positive control: MCF7 cell lysate, HeLa cell lysate, A431 cell lysate, NIH/3T3 cell lysate, Mouse skeletal muscle tissue lysate, Rat skeletal muscle tissue lysate, NIH/3T3.
Subcellular location: Nucleus, Cytoplasm.
Recommended Dilutions:
  WB
  IF-Cell
  FC
1:5,000
1:100
1:1,000
Uniprot #: SwissProt: P51812 Human | P18654 Mouse
Entrez Gene: 501560 Rat
Alternative names: 90 kDa ribosomal protein S6 kinase 3 CLS HU 3 HU2 HU3 Insulin stimulated protein kinase 1 Insulin-stimulated protein kinase 1 ISPK-1 ISPK1 KS6A3_HUMAN MAP kinase activated protein kinase 1b MAP kinase-activated protein kinase 1b MAPK activated protein kinase 1b MAPK-activated protein kinase 1b MAPKAP kinase 1b MAPKAPK 1b MAPKAPK-1b MAPKAPK1B Mental retardation, X linked 19 MRX19 OTTHUMP00000023036 p90 RSK2 p90 RSK3 p90-RSK 3 p90RSK3 pp90RSK2 Ribosomal protein S6 kinase 90kDa polypeptide 3 Ribosomal protein S6 kinase alpha 3 Ribosomal protein S6 kinase alpha-3 Ribosomal protein s6 kinase ii alpha 2 Ribosomal S6 kinase 2 Rps6ka3 RSK RSK-2 RSK2 S6 kinase 2 S6K alpha3 S6K-alpha-3
Images
HA721033_1.jpg Fig1: Western blot analysis of RSK2 on different lysates with Rabbit anti-RSK2 antibody (HA721033) at 1/5,000 dilution.

Lane 1: MCF7 cell lysate (20 µg/Lane)
Lane 2: HeLa cell lysate (20 µg/Lane)
Lane 3: A431 cell lysate (20 µg/Lane)
Lane 4: NIH/3T3 cell lysate (20 µg/Lane)
Lane 5: Mouse skeletal muscle tissue lysate (40 µg/Lane)
Lane 6: Rat skeletal muscle tissue lysate (40 µg/Lane)

Predicted band size: 84 kDa
Observed band size: 75 kDa

Exposure time: 20 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721033) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA721033_2.jpg Fig2: Western blot analysis of RSK2 on different lysates with Rabbit anti-RSK2 antibody (HA721033) at 1/1,000 dilution.

Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-RSK2 KD cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 84 kDa
Observed band size: 75 kDa

Exposure time: 120 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721033) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA721033_3.jpg Fig3: Immunocytochemistry analysis of NIH/3T3 cells labeling RSK2 with Rabbit anti-RSK2 antibody (HA721033) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-RSK2 antibody (HA721033) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
HA721033_4.jpg Fig4: Flow cytometric analysis of NIH/3T3 cells labeling RSK2.

Cells were fixed and permeabilized. Then stained with the primary antibody (HA721033, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.