Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse, Rat |
Applications: | WB, IF-Cell, IHC-P |
Clonality: | Monoclonal |
Clone number: | JE30-36 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 68 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within Human PIAS1 aa 600-651. |
Positive control: | MCF-7 cell lysate, HL-60 cell lysate, rat testis tissue lysate, rat testis tissue, human tonsil tissue, human thyroid tissue, human colon carcinoma tissue, mouse hippocampus tissue, mouse brain tissue, NCI-H441. |
Subcellular location: | Nucleus, Nucleus speckle, Cytoplasm; Nucleus speckle, PML body, Nucleus; Nucleus speckle, PML body. |
Recommended Dilutions:
WB IF-Cell IHC-P |
1:500 1:50 1:100-1:400 |
Uniprot #: | SwissProt: O75925 Human | O88907 Mouse | G3V9T0 Rat |
Alternative names: | AR interacting protein DDXBP1 DEAD/H (Asp-Glu-Ala-Asp/His) box binding protein 1 DEAD/H box binding protein 1 DEAD/H box-binding protein 1 E3 SUMO-protein ligase PIAS1 GBP Gu binding protein Gu-binding protein GU/RH-II Pias1 PIAS1_HUMAN Protein inhibitor of activated STAT protein 1 Protein inhibitor of activated STAT, 1 RNA helicase II binding protein RNA helicase II-binding protein Zinc finger, MIZ-type containing 3 ZMIZ3 |
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Fig1:
Western blot analysis of PIAS1 on different lysates with Rabbit anti-PIAS1 antibody (HA721035) at 1/500 dilution. Lane 1: MCF-7 cell lysate Lane 2: HL-60 cell lysate Lane 3: Rat testis tissue lysate (20 µg/Lane) Lysates/proteins at 10 µg/Lane. Predicted band size: 68 kDa Observed band size: 72 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721035) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-PIAS1 antibody (HA721035) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721035) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-PIAS1 antibody (HA721035) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721035) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human thyroid tissue with Rabbit anti-PIAS1 antibody (HA721035) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721035) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-PIAS1 antibody (HA721035) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721035) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-PIAS1 antibody (HA721035) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721035) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig7:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-PIAS1 antibody (HA721035) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721035) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig8:
Immunocytochemistry analysis of NCI-H441 cells labeling PIAS1 with Rabbit anti-PIAS1 antibody (HA721035) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-PIAS1 antibody (HA721035) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 647, HA1127) were used as the secondary antibody at 1/1,000 dilution. |