PER2 Recombinant Rabbit Monoclonal Antibody [JE33-79]
cat.: HA721036
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE33-79 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 137 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human PER2 aa 50-100. |
| Positive control: | K562 cell lysates, human lung carcinoma tissue, human skin carcinoma tissue. |
| Subcellular location: | Nucleus, Cytoplasm, Perinuclear region; Nucleolus. |
| Recommended Dilutions:
WB IHC-P |
1:500 1:400 |
| Uniprot #: | SwissProt: O15055 Human |
| Alternative names: | Circadian clock protein PERIOD 2 FASPS FASPS1 hPER 2 hPER2 KIAA0347 OTTHUMP00000164476 PER 2 PER2 PER2_HUMAN Period 2 Period 2 isoform 1 Period circadian clock 2 Period circadian protein 2 Period circadian protein homolog 2 Period homolog 2 (Drosophila) Period homolog 2 Period, Drosophila, homolog of, 2 Period2 |
Images
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Fig1:
Western blot analysis of PER2 on K562 cell lysates with Rabbit anti-PER2 antibody (HA721036) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 137 kDa Observed band size: 180 kDa Exposure time: 1 minute; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721036) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-PER2 antibody (HA721036) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721036) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human skin carcinoma tissue with Rabbit anti-PER2 antibody (HA721036) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721036) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.