Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human |
Applications: | WB, IHC-P |
Clonality: | Monoclonal |
Clone number: | JE38-99 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 21 kDa |
Isotype: | IgG |
Immunogen: | Recombinant protein within Human IL23 100-189. |
Positive control: | Daudi cell lysates, THP-1 cell lysate, Raji cell lysate, human lymph nodes tissue. |
Subcellular location: | Secreted. |
Recommended Dilutions:
WB IHC-P |
1:500 1:400 |
Uniprot #: | SwissProt: Q9NPF7 Human |
Alternative names: | IL 23 A IL 23 IL 23 subunit alpha IL 23A IL 23p19 IL-23 subunit alpha IL-23-A IL-23p19 IL12B IL23 Il23a IL23A_HUMAN IL23P19 interleukin 12B Interleukin 23 alpha subunit p19 Interleukin 23 p19 subunit interleukin 23 subunit alpha interleukin 23 subunit p19 interleukin six, G CSF related factor Interleukin-23 subunit alpha Interleukin-23 subunit p19 JKA3 induced upon T cell activation MGC79388 P19 SGRF |
Fig1:
Western blot analysis of IL-23 on Daudi cell lysates with Rabbit anti-IL-23 antibody (HA721037) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 21 kDa Observed band size: 21 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721037) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of IL-23 on different lysates with Rabbit anti-IL-23 antibody (HA721037) at 1/500 dilution. Lane 1: THP-1 cell lysate Lane 2: Raji cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 21 kDa Observed band size: 21 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721037) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
Fig3:
Immunohistochemical analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-IL-23 antibody (HA721037) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721037) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |