IL-23 Recombinant Rabbit Monoclonal Antibody [JE38-99]
cat.: HA721037
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, IHC-P
Clonality: Monoclonal
Clone number: JE38-99
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 21 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human IL23 100-189.
Positive control: Daudi cell lysates, THP-1 cell lysate, Raji cell lysate, human lymph nodes tissue.
Subcellular location: Secreted.
Recommended Dilutions:
  WB
  IHC-P

1:500
1:400
Uniprot #: SwissProt: Q9NPF7 Human
Alternative names: IL 23 A IL 23 IL 23 subunit alpha IL 23A IL 23p19 IL-23 subunit alpha IL-23-A IL-23p19 IL12B IL23 Il23a IL23A_HUMAN IL23P19 interleukin 12B Interleukin 23 alpha subunit p19 Interleukin 23 p19 subunit interleukin 23 subunit alpha interleukin 23 subunit p19 interleukin six, G CSF related factor Interleukin-23 subunit alpha Interleukin-23 subunit p19 JKA3 induced upon T cell activation MGC79388 P19 SGRF
Images
HA721037_1.jpg Fig1: Western blot analysis of IL-23 on Daudi cell lysates with Rabbit anti-IL-23 antibody (HA721037) at 1/500 dilution.

Lysates/proteins at 10 µg/Lane.

Predicted band size: 21 kDa
Observed band size: 21 kDa

Exposure time: 2 minutes;

12% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721037) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
HA721037_2.jpg Fig2: Western blot analysis of IL-23 on different lysates with Rabbit anti-IL-23 antibody (HA721037) at 1/500 dilution.

Lane 1: THP-1 cell lysate
Lane 2: Raji cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 21 kDa
Observed band size: 21 kDa

Exposure time: 2 minutes;

12% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721037) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
HA721037_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-IL-23 antibody (HA721037) at 1/400 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721037) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.