Oncostatin M Recombinant Rabbit Monoclonal Antibody [JE40-04]
cat.: HA721038
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE40-04 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 28 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Oncostatin M aa 26-150. |
| Positive control: | A549 cell lysate, MCF-7 cell lysate, Hela cell lysate, mouse kidney tissue lysate, human colon carcinoma tissue. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IHC-P |
1:500 1:100 |
| Uniprot #: | SwissProt: P13725 Human | P53347 Mouse |
| Alternative names: | MGC20461 ONCM_HUMAN Oncostatin M Oncostatin-M OSM |
Images
|
Fig1:
Western blot analysis of Oncostatin M on different lysates with Rabbit anti-Oncostatin M antibody (HA721038) at 1/500 dilution. Lane 1: A549 cell lysate Lane 2: MCF-7 cell lysate Lane 3: Hela cell lysate Lane 4: Mouse kidney tissue lysate (20 µg/Lane) Lysates/proteins at 10 µg/Lane. Predicted band size: 28 kDa Observed band size: 22 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721038) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Oncostatin M antibody (HA721038) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721038) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.