ITPK1 Recombinant Rabbit Monoclonal Antibody [JE64-35]
cat.: HA721039
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE64-35 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 46 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human ITPK1 aa 362-410. |
| Positive control: | Hela cell lysate, HepG2 cell lysate, 293T cell lysate, A431 cell lysate, MCF-7 cell lysate, human liver tissue. |
| Subcellular location: | Cytosol, apical plasma membrane, cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:5,000 1:100 |
| Uniprot #: | SwissProt: Q13572 Human |
| Alternative names: | 3 4)P(3) 5/6-kinase 4-trisphosphate 5/6-kinase Inositol 1 3 4 triphosphate 5/6 kinase Inositol 1 3 4 trisphosphate 5/6 kinase Inositol 1 Inositol tetrakisphosphate 1 kinase Inositol triphosphate 5/6 kinase Inositol-tetrakisphosphate 1-kinase Inositol-triphosphate 5/6-kinase Ins(1 3 4)P(3) 5/6 kinase Ins(1 ITPK 1 itpk1 ITPK1_HUMAN ITRPK 1 ITRPK1 |
Images
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Fig1:
Western blot analysis of ITPK1 on different lysates with Rabbit anti-ITPK1 antibody (HA721039) at 1/5,000 dilution. Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate Lane 2: Hep G2 (Human hepatocellular carcinoma cell) cell lysate Lane 3: SK-MEL-28 (Human malignant melanoma cell) cell lysate Lane 4: MCF7 (Human breast cancer cell) cell lysate Lane 5: PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 30 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA721039, 1/5,000 in in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 46 kDa Observed band size: 42 kDa |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-ITPK1 antibody (HA721039) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721039) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.