ITPK1 Recombinant Rabbit Monoclonal Antibody [JE64-35]
cat.: HA721039
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE64-35 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 46 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human ITPK1 aa 362-410. |
| Positive control: | Hela cell lysate, HepG2 cell lysate, 293T cell lysate, A431 cell lysate, MCF-7 cell lysate, human liver tissue. |
| Subcellular location: | Cytosol, apical plasma membrane, cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:500 1:100 |
| Uniprot #: | SwissProt: Q13572 Human |
| Alternative names: | 3 4)P(3) 5/6-kinase 4-trisphosphate 5/6-kinase Inositol 1 3 4 triphosphate 5/6 kinase Inositol 1 3 4 trisphosphate 5/6 kinase Inositol 1 Inositol tetrakisphosphate 1 kinase Inositol triphosphate 5/6 kinase Inositol-tetrakisphosphate 1-kinase Inositol-triphosphate 5/6-kinase Ins(1 3 4)P(3) 5/6 kinase Ins(1 ITPK 1 itpk1 ITPK1_HUMAN ITRPK 1 ITRPK1 |
Images
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Fig1:
Western blot analysis of ITPK1 on different lysates with Rabbit anti-ITPK1 antibody (HA721039) at 1/500 dilution. Lane 1: Hela cell lysate Lane 2: HepG2 cell lysate Lane 3: 293T cell lysate Lane 4: A431 cell lysate Lane 5: MCF-7 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 46 kDa Observed band size: 42 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721039) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-ITPK1 antibody (HA721039) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721039) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.