AdSS 2 Recombinant Rabbit Monoclonal Antibody [JE64-42]
cat.: HA721040
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE64-42 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 50 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human AdSS 2 aa 1-200/456. |
| Positive control: | 293T cell lysate, U87MG cell lysate, HL-60 cell lysate, mouse kidney tissue lysate, rat liver tissue, mouse kidney tissue. |
| Subcellular location: | Cytoplasm, Mitochondrion. |
| Recommended Dilutions:
WB IHC-P |
1:500 1:100-1:400 |
| Uniprot #: | SwissProt: P30520 Human | P46664 Mouse | D4AEP0 Rat |
| Alternative names: | (Ade(-)H, complement of acidic isozyme ADEH Adenylosuccinate synthetase (Ade(-)H complementing) Adenylosuccinate synthetase acidic isozyme Adenylosuccinate synthetase Adenylosuccinate synthetase isozyme 2 Adenylosuccinate synthetase liver isozyme AdSS 2 ADSS AMPSase 2 IMP--aspartate ligase 2 L-type adenylosuccinate synthetase liver isozyme PURA2_HUMAN |
Images
|
Fig1:
Western blot analysis of AdSS 2 on different lysates with Rabbit anti-AdSS 2 antibody (HA721040) at 1/500 dilution. Lane 1: 293T cell lysate Lane 2: U87MG cell lysate Lane 3: HL-60 cell lysate Lane 4: Mouse kidney tissue lysate (20 µg/Lane) Lysates/proteins at 10 µg/Lane. Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721040) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-AdSS 2 antibody (HA721040) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721040) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-AdSS 2 antibody (HA721040) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721040) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.