IQGAP2 Recombinant Rabbit Monoclonal Antibody [JE64-54]
cat.: HA721041
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE64-54 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 181 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human IQGAP2 aa 1,301-1,500/1,575. |
| Positive control: | Human placenta tissue lysates, human gallbladder tissue, human prostate tissue. |
| Subcellular location: | Actin cytoskeleton, microtubule, cytosol, extracellular exosome, plasma membrane, cell surface, cytoplasm, filopodium, lamellipodium, microvillus, secretory granule membrane |
| Recommended Dilutions:
WB IHC-P |
1:1,000-1:2,000 1:100 |
| Uniprot #: | SwissProt: Q13576 Human |
| Alternative names: | IQ motif containing GTPase activating protein 2 IQGA2_HUMAN Iqgap2 Ras GTPase activating-like protein Ras GTPase-activating-like protein IQGAP2 |
Images
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Fig1:
Western blot analysis of IQGAP2 on human placenta tissue lysates with Rabbit anti-IQGAP2 antibody (HA721041) at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 181 kDa Observed band size: 181 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721041) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of IQGAP2 on different lysates with Rabbit anti-IQGAP2 antibody (HA721041) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-IQGAP2 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 181 kDa Observed band size: 181 kDa Exposure time: 120 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721041) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human gallbladder tissue with Rabbit anti-IQGAP2 antibody (HA721041) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721041) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded human prostate tissue with Rabbit anti-IQGAP2 antibody (HA721041) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721041) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.