PNKP Recombinant Rabbit Monoclonal Antibody [JE64-21]
cat.: HA721044
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE64-21 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 57 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human PNKP aa 51-100 / 521. |
| Positive control: | Jurkat cell lysate, HepG2 cell lysate, PC-3 cell lysate, Hela cell lysate, human breast carcinoma tissue, human kidney tissue, Hela. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:500-1,000 1:100 1:400 |
| Uniprot #: | SwissProt: Q96T60 Human |
| Alternative names: | 2''(3'')-polynucleotidase 2'(3')-polynucleotidase Bifunctional polynucleotide phosphatase/kinase DEM 1 DEM1 DNA 5' kinase/3' phosphatase DNA 5''-kinase/3''-phosphatase EIEE10 Homo sapiens polynucleotide kinase 3' phosphatase (PNKP) MCSZ PNK 1 PNK1 Pnkp PNKP DNA kinase PNKP_HUMAN Polynucleotide 3'-phosphatase Polynucleotide 5' hydroxyl kinase Polynucleotide 5''-hydroxyl-kinase Polynucleotide kinase 3 prime phosphatase Polynucleotide kinase 3' phosphatase Polynucleotide Kinase Polynucleotide kinase-3''-phosphatase |
Images
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Fig1:
Western blot analysis of PNKP on different lysates with Rabbit anti-PNKP antibody (HA721044) at 1/1,000 dilution. Lane 1: HeLa Lane 2: Jurkat Lane 3: Hep G2 Lysates/proteins at 20 µg/Lane. Exposure time: 56 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA721044, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 57.1 kDa Observed band size: 60 kDa |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-PNKP antibody (HA721044) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721044) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-PNKP antibody (HA721044) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721044) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunocytochemistry analysis of Hela cells labeling PNKP with Rabbit anti-PNKP antibody (HA721044) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-PNKP antibody (HA721044) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (Alexa Fluor® 555) were used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.