KIF5B Recombinant Rabbit Monoclonal Antibody [JE64-16]
cat.: HA721045
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE64-16 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 110 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human KIF5B aa 1-50. |
| Positive control: | HeLa cell lysate, A549 cell lysate, mouse kidney tissue lysate, HCT116 cell lysate, C2C12 cell lysate, MCF-7 cell lysate, Hela cell lysate, HepG2 cell lysate, mouse brain tissue lysate, rat brain tissue lysate, human gallbladder tissue, human testis tissue, rat uterus tissue, rat cerebellum tissue lysate, rat bladder tissue lysate. |
| Subcellular location: | Cytolytic granule membrane, Lysosome membrane, cytoskeleton. |
| Recommended Dilutions:
WB IHC-P |
1:500 1:100-1:400 |
| Uniprot #: | SwissProt: P33176 Human | Q61768 Mouse | Q2PQA9 Rat |
| Alternative names: | Conventional kinesin heavy chain KIF 5B KIF5B Kinesin 1 kinesin 1 (110-120kD) Kinesin 1 heavy chain Kinesin family member 5B Kinesin heavy chain kinesin, heavy chain, ubiquitous Kinesin-1 heavy chain Kinesin1 KINH KINH_HUMAN KNS 1 KNS KNS1 Ubiquitous kinesin heavy chain UKHC |
Images
|
Fig1:
Western blot analysis of KIF5B on different lysates with Rabbit anti-KIF5B antibody at 1/500 dilution. Lane 1: HeLa cell lysate, 10 µg/Lane Lane 2: A549 cell lysate, 10 µg/Lane Lane 3: Mouse kidney tissue lysate, 20 µg/Lane Predicted band size: 110 kDa Observed band size: 110 kDa Exposure time: 1 minute; 8 % SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody KIF5B at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of KIF5B on different lysates with Rabbit anti-KIF5B antibody at 1/500 dilution. Lane 1: HCT116 cell lysate Lane 2: C2C12 cell lysate Lane 3: MCF-7 cell lysate Lane 4: Hela cell lysate Lane 5: HepG2 cell lysate Lane 6: Mouse brain tissue lysate (20 µg/Lane) Lane 7: Rat brain tissue lysate (20 µg/Lane) Lysates/proteins at 10 µg/Lane. Predicted band size: 110 kDa Observed band size: 110 kDa Exposure time: 1 minute; 8 % SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody KIF5B at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human gallbladder tissue with Rabbit anti-KIF5B antibody (HA721045) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody KIF5B at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-KIF5B antibody (HA721045) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody KIF5B at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Immunohistochemical analysis of paraffin-embedded rat uterus tissue with Rabbit anti-KIF5B antibody (HA721045) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody KIF5B at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig6:
Western blot analysis of KIF5B on different lysates with Rabbit anti-KIF5B antibody (HA721045) at 1/500 dilution. Lane 1: Rat cerebellum tissue lysate Lane 2: Rat bladder tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 110 kDa Observed band size: 110 kDa Exposure time: 1 minute; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721045) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.