PPM1G Recombinant Rabbit Monoclonal Antibody [JE64-91]
cat.: HA721046
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JE64-91 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 59 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human PPM1G aa 301-500/546. |
| Positive control: | 293T cell lysate, Jurkat cell lysate, rat testis tissue lysate, rat large intestine tissue, human testis tissue, human colon carcinoma tissue, human small intestine tissue, HepG2. |
| Subcellular location: | Cytoplasm, Membrane. |
| Recommended Dilutions:
WB IHC-P FC |
1:500 1:100-1:400 1:500-1:1,000 |
| Uniprot #: | SwissProt: O15355 Human | Q61074 Mouse Entrez Gene: 259229 Rat |
| Alternative names: | MGC1675 MGC2870 OTTHUMP00000123426 PP2C PP2C gamma PP2C-gamma PP2CG PP2Cgamma PPM1G PPM1G_HUMAN PPP2CG Protein phosphatase 1C Protein phosphatase 1G Protein phosphatase 2 catalytic subunit gamma isoform Protein phosphatase 2C gamma isoform Protein phosphatase 2C isoform gamma Protein phosphatase magnesium dependent 1 gamma Protein phosphatase magnesium-dependent 1 gamma Protein phosphatase Mg2+/Mn2+ dependent 1G |
Images
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Fig1:
Western blot analysis of PPM1G on different lysates with Rabbit anti-PPM1G antibody (HA721046) at 1/500 dilution. Lane 1: 293T cell lysate, 10 µg/Lane Lane 2: Jurkat cell lysate, 10 µg/Lane Lane 3: Rat testis tissue lysate, 20 µg/Lane Predicted band size: 59 kDa Observed band size: 70 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721046) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded rat large intestine tissue with Rabbit anti-PPM1G antibody (HA721046) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721046) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-PPM1G antibody (HA721046) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721046) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-PPM1G antibody (HA721046) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721046) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-PPM1G antibody (HA721046) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721046) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Flow cytometric analysis of HepG2 cells labeling PPM1G. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721046, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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