AK2 Recombinant Rabbit Monoclonal Antibody [JE64-27]
cat.: HA721048
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE64-27 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 26 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human AK2 aa 150-200/239. |
| Positive control: | A549 cell lysate, HL-60 cell lysate, HepG2 cell lysate, Mouse liver cell lysate, Mouse kidney cell lysate, Rat liver cell lysate, Rat kidney cell lysate, human kidney tissue. |
| Subcellular location: | Mitochondrion intermembrane space. |
| Recommended Dilutions:
WB IHC-P |
1:1,000-1:5,000 1:100 |
| Uniprot #: | SwissProt: P54819 Human | Q9WTP6 Mouse | P29410 Rat |
| Alternative names: | Adenylate kinase 2 adenylate kinase 2, mitochondrial Adenylate kinase isoenzyme 2 ADK2 AK 2 ak2 ATP AMP transphosphorylase ATP-AMP transphosphorylase 2 EC 2.7.4.3 KAD2_HUMAN mitochondrial |
Images
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Fig1:
Western blot analysis of AK2 on different lysates with Rabbit anti-AK2 antibody at 1/1,000 dilution. Lane 1: A549 cell lysate Lane 2: HL-60 cell lysate Lane 3: HepG2 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 26 kDa Observed band size: 26 kDa Exposure time: 2 minutes; 12 % SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody AK2 at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of AK2 on different lysates with Rabbit anti-AK2 antibody at 1/5,000 dilution. Lane 1: Mouse liver cell lysate Lane 2: Mouse kidney cell lysate Lane 3: Rat liver cell lysate Lane 4: Rat kidney cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 26 kDa Observed band size: 26 kDa Exposure time: 2 minutes; 12 % SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody AK2 at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-AK2 antibody (HA721048) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody AK2 at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.