AK2 Recombinant Rabbit Monoclonal Antibody [JE64-27]
cat.: HA721048
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | JE64-27 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 26 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human AK2 aa 150-200/239. |
| Positive control: | A549 cell lysate, HL-60 cell lysate, HepG2 cell lysate, Mouse liver cell lysate, Mouse kidney cell lysate, Rat liver cell lysate, Rat kidney cell lysate, human kidney tissue, A375 . |
| Subcellular location: | Mitochondrion intermembrane space. |
| Recommended Dilutions:
WB IHC-P IF-Cell |
1:1,000-1:5,000 1:100 1:50 |
| Uniprot #: | SwissProt: P54819 Human | Q9WTP6 Mouse | P29410 Rat |
| Alternative names: | Adenylate kinase 2 adenylate kinase 2, mitochondrial Adenylate kinase isoenzyme 2 ADK2 AK 2 ak2 ATP AMP transphosphorylase ATP-AMP transphosphorylase 2 EC 2.7.4.3 KAD2_HUMAN mitochondrial |
Images
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Fig1:
Western blot analysis of AK2 on different lysates with Rabbit anti-AK2 antibody at 1/1,000 dilution. Lane 1: A549 cell lysate Lane 2: HL-60 cell lysate Lane 3: HepG2 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 26 kDa Observed band size: 26 kDa Exposure time: 2 minutes; 12 % SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody AK2 at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of AK2 on different lysates with Rabbit anti-AK2 antibody at 1/5,000 dilution. Lane 1: Mouse liver cell lysate Lane 2: Mouse kidney cell lysate Lane 3: Rat liver cell lysate Lane 4: Rat kidney cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 26 kDa Observed band size: 26 kDa Exposure time: 2 minutes; 12 % SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody AK2 at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-AK2 antibody (HA721048) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody AK2 at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunocytochemistry analysis of A375 cells labeling AK2 with Rabbit anti-AK2 antibody (HA721048) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-AK2 antibody (HA721048) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.