TPMT Recombinant Rabbit Monoclonal Antibody [JE64-09]
cat.: HA721049
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE64-09 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 28 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human TPMT aa 101-150/245. |
| Positive control: | K-562 cell lysate, SK-Br-3 cell lysate, Mouse brain tissue lysate, Mouse kidney tissue lysate, Rat lung tissue lysate, Rat kidney tissue lysate, mouse hippocampus tissue, mouse brain tissue. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:5,000 1:400-1:1,000 |
| Uniprot #: | SwissProt: P51580 Human | O55060 Mouse | Q9Z0T0 Rat |
| Alternative names: | HGNC:12014 S adenosyl L methionine thiopurine S methyltransferase Thiopurine methyltransferase Thiopurine S methyltransferase Thiopurine S-methyltransferase TPMT TPMT_HUMAN |
Images
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Fig1:
Western blot analysis of TPMT on different lysates with Rabbit anti-TPMT antibody (HA721049) at 1/5,000 dilution. Lane 1: K-562 cell lysate (10 µg/Lane) Lane 2: SK-Br-3 cell lysate (10 µg/Lane) Lane 3: Mouse brain tissue lysate (20 µg/Lane) Lane 4: Mouse kidney tissue lysate (20 µg/Lane) Lane 5: Rat lung tissue lysate (20 µg/Lane) Lane 6: Rat kidney tissue lysate (20 µg/Lane) Predicted band size: 28 kDa Observed band size: 28 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721049) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of TPMT on different lysates with Rabbit anti-TPMT antibody (HA721049) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-TPMT KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 28 kDa Observed band size: 28 kDa Exposure time: 120 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721049) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.