TPMT Recombinant Rabbit Monoclonal Antibody [JE64-09]
cat.: HA721049
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JE64-09 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 28 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human TPMT aa 101-150/245. |
| Positive control: | K562 cell lysate, SK-Br-3 cell lysate, HepG2 cell lysate, A431 cell lysate, mouse brain tissue lysate, rat stomach tissue lysate, mouse kidney tissue lysate, rat lung tissue lysate, mouse hippocampus tissue, K562, mouse brain tissue. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IHC-P FC |
1:1,000-1:2,000 1:400-1:1,000 1:500-1:1,000 |
| Uniprot #: | SwissProt: P51580 Human | O55060 Mouse | Q9Z0T0 Rat |
| Alternative names: | HGNC:12014 S adenosyl L methionine thiopurine S methyltransferase Thiopurine methyltransferase Thiopurine S methyltransferase Thiopurine S-methyltransferase TPMT TPMT_HUMAN |
Images
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Fig1:
Western blot analysis of TPMT on different lysates with Rabbit anti-TPMT antibody (HA721049) at 1/500 dilution. Lane 1: K562 cell lysate Lane 2: SK-Br-3 cell lysate Lane 3: HepG2 cell lysate Lane 4: A431 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 28 kDa Observed band size: 28 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721049) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of TPMT on different lysates with Rabbit anti-TPMT antibody (HA721049) at 1/500 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat stomach tissue lysate Lane 3: Mouse kidney tissue lysate Lane 4: Rat lung tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 28 kDa Observed band size: 28 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721049) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-TPMT antibody (HA721049) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721049) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Flow cytometric analysis of K562 cells labeling TPMT. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721049, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig5:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-TPMT antibody (HA721049) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721049) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Western blot analysis of TPMT on different lysates with Rabbit anti-TPMT antibody (HA721049) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-TPMT KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 28 kDa Observed band size: 28 kDa Exposure time: 120 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721049) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.