ACTR1B Recombinant Rabbit Monoclonal Antibody [JE65-44]
cat.: HA721050
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JE65-44 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 42 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human ACTR1B aa 277-376/376. |
| Positive control: | HepG2 cell lysates, Jurkat cell lysate, Hela cell lysate, human liver tissue lysate, human lung tissue lysate, human colon carcinoma tissue, human ovarian carcinoma tissue, HepG2. |
| Subcellular location: | Cytoskeleton, centrosome. |
| Recommended Dilutions:
WB IHC-P FC |
1:1,000 1:400 1:500-1:1,000 |
| Uniprot #: | SwissProt: P42025 Human |
| Alternative names: | Actin related protein 1B Actin-related protein 1B ACTR 1B ACTR1B ACTY_HUMAN ARP 1B ARP1 actin related protein 1 homolog B ARP1 actin related protein 1 homolog B centractin beta (yeast) ARP1 actin related protein 1 homolog B centractin beta ARP1 actin related protein 1 yeast homolog B centractin beta ARP1 yeast homolog B ARP1B Beta centractin Beta-centractin Centractin beta (Yeast) Centractin beta CTRN 2 CTRN2 PC 3 PC3 |
Images
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Fig1:
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-ACTR1B antibody (HA721050) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721050) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma tissue with Rabbit anti-ACTR1B antibody (HA721050) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721050) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Flow cytometric analysis of HepG2 cells labeling ACTR1B. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721050, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
|
Fig4:
Western blot analysis of ACTR1B on different lysates with Rabbit anti-ACTR1B antibody (HA721050) at 1/2,000 dilution. Lane 1: HepG2(Human liver cancer cellsl)cell lysate Lane 2: Jurkat(Human T-lymphocyte leukemia cells)cell lysate Lane 3: HL-60 (Human early lymphoblastic leukemia cells) cell lysate Lane 4: SK-OV-3(Human ovarian adenocarcinoma cells) cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 20 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA721050, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 42.3 kDa Observed band size: 42 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.