ACTR1B Recombinant Rabbit Monoclonal Antibody [JE65-44]
cat.: HA721050
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JE65-44 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 42 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human ACTR1B aa 277-376/376. |
| Positive control: | HepG2 cell lysates, Jurkat cell lysate, Hela cell lysate, human liver tissue lysate, human lung tissue lysate, human colon carcinoma tissue, human ovarian carcinoma tissue, HepG2. |
| Subcellular location: | Cytoskeleton, centrosome. |
| Recommended Dilutions:
WB IHC-P FC |
1:1,000 1:400 1:500-1:1,000 |
| Uniprot #: | SwissProt: P42025 Human |
| Alternative names: | Actin related protein 1B Actin-related protein 1B ACTR 1B ACTR1B ACTY_HUMAN ARP 1B ARP1 actin related protein 1 homolog B ARP1 actin related protein 1 homolog B centractin beta (yeast) ARP1 actin related protein 1 homolog B centractin beta ARP1 actin related protein 1 yeast homolog B centractin beta ARP1 yeast homolog B ARP1B Beta centractin Beta-centractin Centractin beta (Yeast) Centractin beta CTRN 2 CTRN2 PC 3 PC3 |
Images
|
Fig1:
Western blot analysis of ACTR1B on HepG2 cell lysates with Rabbit anti-ACTR1B antibody (HA721050) at 1/1,000 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721050) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of ACTR1B on different lysates with Rabbit anti-ACTR1B antibody (HA721050) at 1/1,000 dilution. Lane 1: Jurkat cell lysate, 10 µg/Lane Lane 2: Hela cell lysate, 10 µg/Lane Lane 3: Human liver tissue lysate, 20 µg/Lane Lane 4: Human lung tissue lysate, 20 µg/Lane Lysates/proteins at 10 µg/Lane. Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721050) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-ACTR1B antibody (HA721050) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721050) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma tissue with Rabbit anti-ACTR1B antibody (HA721050) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721050) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Flow cytometric analysis of HepG2 cells labeling ACTR1B. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721050, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.