MYO19 Recombinant Rabbit Monoclonal Antibody [JE64-30]
cat.: HA721051
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | JE64-30 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 109 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human MYO19 aa 431-680/970. |
| Positive control: | NCI-H441, RAW264.7 cell lysate, NIH/3T3 cell lysate, C6 cell lysate. |
| Subcellular location: | Cytoskeleton, Mitochondrion outer membrane. |
| Recommended Dilutions:
WB IF-Cell |
1:5,000 1:50 |
| Uniprot #: | SwissProt: Q96H55 Human |
| Alternative names: | FLJ22865 myo19 MYO19_HUMAN Myohd1 Myosin head domain containing 1 Myosin head domain-containing protein 1 myosin XIX Myosin-XIX unconventional myosin-XIX |
Images
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Fig1:
Immunocytochemistry analysis of NCI-H441 cells labeling MYO19 with Rabbit anti-MYO19 antibody (HA721051) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-MYO19 antibody (HA721051) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 647, HA1127) were used as the secondary antibody at 1/1,000 dilution. |
|
Fig2:
Western blot analysis of MYO19 on different lysates with Rabbit anti-MYO19 antibody (HA721051) at 1/5,000 dilution. Lane 1: RAW264.7 cell lysate Lane 2: NIH/3T3 cell lysate Lane 3: C6 cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 59 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA721051, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 109 kDa Observed band size: 109 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.