TRIM24 Recombinant Rabbit Monoclonal Antibody [JE64-31]
cat.: HA721052
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE64-31 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 117 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human TRIM24 aa 405-455/1,050. |
| Positive control: | HepG2 cell lysate, HeLa cell lysate, human bladder carcinoma tissue, human breast carcinoma tissue, human thyroid tissue. |
| Subcellular location: | Nucleus, Cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:500 1:400-1:2,000 |
| Uniprot #: | SwissProt: O15164 Human |
| Alternative names: | E3 ubiquitin protein ligase TRIM24 E3 ubiquitin-protein ligase Trim24 hTIF1 PTC6 RING finger protein 82 RNF82 TF1A TIF1 alpha TIF1 TIF1-alpha TIF1A TIF1A_HUMAN TIF1ALPHA Transcription intermediary factor 1-alpha Transcriptional intermediary factor 1 alpha Transcriptional intermediary factor 1 Trim24 Tripartite motif containing 24 Tripartite motif-containing protein 24 |
Images
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Fig1:
Western blot analysis of TRIM24 on different lysates with Rabbit anti-TRIM24 antibody at 1/500 dilution. Lane 1: HepG2 cell lysate Lane 2: HeLa cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 117 kDa Observed band size: 130 kDa Exposure time: 2 minutes; 6 % SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody TRIM24 at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human bladder carcinoma tissue with Rabbit anti-TRIM24 antibody at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody TRIM24 at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-TRIM24 antibody at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody TRIM24 at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded human thyroid tissue with Rabbit anti-TRIM24 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody TRIM24 at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.