WDR1 Recombinant Rabbit Monoclonal Antibody [JE64-32]
cat.: HA721053
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE64-32 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 66 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human WDR1 aa 301-350/606. |
| Positive control: | HeLa cell lysate, Raji cell lysate, NIH/3T3 cell lysate, C6 cell lysate, Rat skeletal muscle tissue lysate, human gastric tissue, human endometrial tissue. |
| Subcellular location: | Cytoskeleton, Cytoplasm, podosome, Cell junction. |
| Recommended Dilutions:
WB IHC-P |
1:2,000 1:100-1:400 |
| Uniprot #: | SwissProt: O75083 Human | O88342 Mouse | Q5RKI0 Rat |
| Alternative names: | Actin interacting protein 1 Actin-interacting protein 1 AIP1 D5Wsu185e NORI 1 NORI-1 WD repeat domain 1 WD repeat-containing protein 1 wdr1 WDR1_HUMAN |
Images
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Fig1:
Western blot analysis of WDR1 on different lysates with Rabbit anti-WDR1 antibody (HA721053) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: Raji cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: C6 cell lysate Lane 5: Rat skeletal muscle tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 66 kDa Observed band size: 75 kDa Exposure time: Lane 1-4: 23 seconds; Lane 5: 1 minute 7 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721053) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human gastric tissue with Rabbit anti-WDR1 antibody at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody WDR1 at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human endometrial tissue with Rabbit anti-WDR1 antibody at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody WDR1 at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.