AAMP Recombinant Rabbit Monoclonal Antibody [JE64-70]
cat.: HA721054
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC |
| Clonality: | Monoclonal |
| Clone number: | JE64-70 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 47 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human AAMP aa 2-51/434. |
| Positive control: | HepG2 cell lysate, 293T cell lysate, rat testis tissue lysate, MCF-7 cell lysate, HUVEC cell lysate, NIH/3T3 cell lysate, rat brain tissue lysate, HepG2. |
| Subcellular location: | Cell membrane, Cytoplasm. |
| Recommended Dilutions:
WB FC |
1:500-1:2,000 1:500-1:1,000 |
| Uniprot #: | SwissProt: Q13685 Human Entrez Gene: 227290 Mouse | 301512 Rat |
| Alternative names: | AAMP Angio associated migratory cell protein |
Images
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Fig1:
Western blot analysis of AAMP on different lysates with Rabbit anti-AAMP antibody (HA721054) at 1/2,000 dilution. Lane 1: HepG2 cell lysate, 10 µg/Lane Lane 2: 293T cell lysate, 10 µg/Lane Lane 3: Rat testis tissue lysate, 20 µg/Lane Predicted band size: 47 kDa Observed band size: 50 kDa Exposure time: 1 minute; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721054) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of AAMP on different lysates with Rabbit anti-AAMP antibody (HA721054) at 1/500 dilution. Lane 1: MCF-7 cell lysate, 10 µg/Lane Lane 2: HUVEC cell lysate, 10 µg/Lane Lane 3: NIH/3T3 cell lysate, 10 µg/Lane Lane 4: Rat brain tissue lysate, 20 µg/Lane Predicted band size: 47 kDa Observed band size: 50 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721054) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Flow cytometric analysis of HepG2 cells labeling AAMP. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721054, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.