Septin 8 Recombinant Rabbit Monoclonal Antibody [JE64-99]
cat.: HA721055
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE64-99 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 56 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human Septin 8 aa 41-90/483. |
| Positive control: | K562 cell lysate, HCT 116 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysates, mouse hippocampus tissue, mouse brain tissue, rat cerebellum tissue, rat brain tissue. |
| Subcellular location: | Cytoskeleton, cytoplasm, synapse, axon, synaptic vesicle membrane, presynapse. |
| Recommended Dilutions:
WB IHC-P |
1:5,000 1:200-1:500 |
| Uniprot #: | SwissProt: Q92599 Human | Q8CHH9 Mouse | B0BNF1 Rat |
| Alternative names: | KIAA0202 OTTHUMP00000211755 SEP 2 SEP2 SEPT 8 SEPT8 Septin8 |
Images
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Fig1:
Western blot analysis of Septin 8 on different lysates with Rabbit anti-Septin 8 antibody (HA721055) at 1/5,000 dilution. Lane 1: K-562 (Human chronic myelogenous leukemia cell) cell lysate Lane 2: HCT 116 (Human colon cancer cell) cell lysate Lane 3: NIH/3T3 (Mouse fibroblast) cell lysate Lane 4: PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 20 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA721055, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 56 kDa Observed band size: 51 kDa |
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Fig2:
Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-Septin 8 antibody (HA721055) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721055) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Septin 8 antibody (HA721055) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721055) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-Septin 8 antibody (HA721055) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721055) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-Septin 8 antibody (HA721055) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721055) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.