EPHX2 Recombinant Rabbit Monoclonal Antibody [JE64-07]
cat.: HA721056
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB
Clonality: Monoclonal
Clone number: JE64-07
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 63 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human EPHX2 aa 1-50/555.
Positive control: Jurkat cell lysate, SH-SY5Y cell lysate.
Subcellular location: Peroxisome, Cytoplasm.
Recommended Dilutions:
  WB

1:500
Uniprot #: SwissProt: P34913 Human
Alternative names: Bifunctional epoxide hydrolase 2 CEH Cytosolic epoxide hydrolase EPHX2 Epoxide hydratase Epoxide hydrolase 2 Epoxide hydrolase 2 cytoplasmic epoxide hydrolase 2, cytosolic Epoxide hydrolase soluble HYES_HUMAN SEH Soluble epoxide hydrolase
Images
HA721056_1.jpg Fig1: Western blot analysis of EPHX2 on different lysates with Rabbit anti-EPHX2 antibody (HA721056) at 1/500 dilution.

Lane 1: Jurkat cell lysate
Lane 2: SH-SY5Y cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 63 kDa
Observed band size: 70 kDa

Exposure time: 1 minute; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721056) at 1/500 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.