EPHX2 Recombinant Rabbit Monoclonal Antibody [JE64-07]
cat.: HA721056
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE64-07 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 63 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human EPHX2 aa 1-50/555. |
| Positive control: | Jurkat cell lysate, HepG2cell lysate, Hela cell lysate. |
| Subcellular location: | Peroxisome, Cytoplasm. |
| Recommended Dilutions:
WB |
1:500 |
| Uniprot #: | SwissProt: P34913 Human |
| Alternative names: | Bifunctional epoxide hydrolase 2 CEH Cytosolic epoxide hydrolase EPHX2 Epoxide hydratase Epoxide hydrolase 2 Epoxide hydrolase 2 cytoplasmic epoxide hydrolase 2, cytosolic Epoxide hydrolase soluble HYES_HUMAN SEH Soluble epoxide hydrolase |
Images
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Fig1:
Western blot analysis of EPHX2 on different lysates with Rabbit anti-EPHX2 antibody (HA721056) at 1/500 dilution. Lane 1: Jurkat cell lysate Lane 2: HepG2cell lysate Lane 3: Hela cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 63 kDa Observed band size: 65 kDa Exposure time: 30 seconds; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721056) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.