UFD1L Recombinant Rabbit Monoclonal Antibody [JE64-80]
cat.: HA721057
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE64-80 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 35 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human UFD1L aa 11-60/307. |
| Positive control: | MCF7 cell lysate, MOLT-4 cell lysate, PC-12 cell lysate, Mouse brain tissue lysate, Rat testis tissue lysate. |
| Subcellular location: | Nucleus, cytosol. |
| Recommended Dilutions:
WB |
1:5,000 |
| Uniprot #: | SwissProt: Q92890 Human | P70362 Mouse | Q9ES53 Rat |
| Alternative names: | UB fusion protein 1 Ubiquitin fusion degradation 1 like (yeast) Ubiquitin fusion degradation 1 like Ubiquitin fusion degradation protein 1 homolog UFD1 UFD1_HUMAN UFD1L |
Images
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Fig1:
Western blot analysis of UFD1L on different lysates with Rabbit anti-UFD1L antibody (HA721057) at 1/5,000 dilution. Lane 1: MCF7 cell lysate (10 µg/Lane) Lane 2: MOLT-4 cell lysate (10 µg/Lane) Lane 3: PC-12 cell lysate (10 µg/Lane) Lane 4: Mouse brain tissue lysate (20 µg/Lane) Lane 5: Rat testis tissue lysate (20 µg/Lane) Predicted band size: 35 kDa Observed band size: 39 kDa Exposure time: 14 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721057) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.