UFD1L Recombinant Rabbit Monoclonal Antibody [JE64-80]
cat.: HA721057
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P
Clonality: Monoclonal
Clone number: JE64-80
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 35 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human UFD1L aa 11-60/307.
Positive control: MCF7 cell lysate, MOLT-4 cell lysate, PC-12 cell lysate, Mouse brain tissue lysate, Rat testis tissue lysate, rat skeletal muscle tissue, human cervix tissue, mouse skeletal muscle tissue.
Subcellular location: Nucleus, cytosol.
Recommended Dilutions:
  WB
  IHC-P
1:5,000
1:100
Uniprot #: SwissProt: Q92890 Human | P70362 Mouse | Q9ES53 Rat
Alternative names: UB fusion protein 1 Ubiquitin fusion degradation 1 like (yeast) Ubiquitin fusion degradation 1 like Ubiquitin fusion degradation protein 1 homolog UFD1 UFD1_HUMAN UFD1L
Images
HA721057_1.jpg Fig1: Western blot analysis of UFD1L on different lysates with Rabbit anti-UFD1L antibody (HA721057) at 1/5,000 dilution.

Lane 1: MCF7 cell lysate (10 µg/Lane)
Lane 2: MOLT-4 cell lysate (10 µg/Lane)
Lane 3: PC-12 cell lysate (10 µg/Lane)
Lane 4: Mouse brain tissue lysate (20 µg/Lane)
Lane 5: Rat testis tissue lysate (20 µg/Lane)

Predicted band size: 35 kDa
Observed band size: 39 kDa

Exposure time: 14 seconds; ECL: K1801;
4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721057) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA721057_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue with Rabbit anti-UFD1L antibody (HA721057) at 1/100 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721057) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721057_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human cervix tissue with Rabbit anti-UFD1L antibody (HA721057) at 1/100 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721057) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721057_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue with Rabbit anti-UFD1L antibody (HA721057) at 1/100 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721057) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.