ACADS Recombinant Rabbit Monoclonal Antibody [JE64-13]
cat.: HA721058
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE64-13 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 44 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human ACADS aa 363-412/412. |
| Positive control: | Hela cell lysate, 293T cell lysate, mouse liver tissue lysate, rat kidney tissue lysate, human liver tissue, human colon carcinoma tissue. |
| Subcellular location: | Mitochondrion matrix. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:2,000 1:100 |
| Uniprot #: | SwissProt: P16219 Human | Q07417 Mouse | P15651 Rat |
| Alternative names: | ACAD3 ACADS ACADS_HUMAN Acyl Coenzyme A dehydrogenase, C2 to C3 short chain Acyl-CoA dehydrogenase, C2 to C3 short chain Acyl-CoA dehydrogenase, short chain Acyl-Coenzyme A dehydrogenase, short chain AI196007 Bcd-1 Bcd1 Butyryl CoA dehydrogenase Butyryl-CoA dehydrogenase EC 1.3.99.2 mitochondrial SCAD Short chain acyl CoA dehydrogenase Short-chain specific acyl-CoA dehydrogenase Short-chain specific acyl-CoA dehydrogenase, mitochondrial Unsaturated acyl CoA reductase |
Images
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Fig1:
Western blot analysis of ACADS on different lysates with Rabbit anti-ACADS antibody (HA721058) at 1/2,000 dilution. Lane 1: A549-si NT cell lysate Lane 2: A549-si ACADS cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 44 kDa Observed band size: 44 kDa Exposure time: 2 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721058) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of ACADS on different lysates with Rabbit anti-ACADS antibody (HA721058) at 1/500 dilution. Lane 1: Hela cell lysate Lane 2: 293T cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 44 kDa Observed band size: 39 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721058) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Western blot analysis of ACADS on different lysates with Rabbit anti-ACADS antibody (HA721058) at 1/1,000 dilution. Lane 1: Mouse liver tissue lysate Lane 2: Rat kidney tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 44 kDa Observed band size: 39 kDa Exposure time: 30 seconds; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721058) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-ACADS antibody (HA721058) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721058) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-ACADS antibody (HA721058) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721058) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.