EF-Ts Recombinant Rabbit Monoclonal Antibody [JE64-29]
cat.: HA721059
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE64-29 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 35 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human EF-Ts aa 1-100/325. |
| Positive control: | HepG2 cell lysate, Jurkat cell lysate, K562 cell lysate, mouse liver tissue lysate, mouse kidney tissue lysate, Hela cell lysates, human kidney tissue. |
| Subcellular location: | Mitochondrion. |
| Recommended Dilutions:
WB IHC-P |
1:500 1:100 |
| Uniprot #: | SwissProt: P43897 Human | Q9CZR8 Mouse |
| Alternative names: | COXPD 3 COXPD3 EF TS EF Tsmt EF-Ts EF-TsMt EFTS EFTS_HUMAN EFTSMT Elongation factor Ts Elongation factor Ts mitochondrial HGNC12367 mitochondrial Mitochondrial elongation factor Ts Ts translation elongation factor mitochondrial TSFM TSMT |
Images
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Fig1:
Western blot analysis of EF-Ts on different lysates with Rabbit anti-EF-Ts antibody (HA721059) at 1/500 dilution. Lane 1: HepG2 cell lysate, 10 µg/Lane Lane 2: Jurkat cell lysate, 10 µg/Lane Lane 3: K562 cell lysate, 10 µg/Lane Lane 4: Mouse liver tissue lysate, 20 µg/Lane Lane 5: Mouse kidney tissue lysate, 20 µg/Lane Predicted band size: 35 kDa Observed band size: 35 kDa Exposure time: 1 minute; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721059) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of EF-Ts on Hela cell lysates with Rabbit anti-EF-Ts antibody (HA721059) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 35 kDa Observed band size: 35 kDa Exposure time: 1 minute; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721059) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-EF-Ts antibody (HA721059) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721059) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.