HA721060

USO1 Recombinant Rabbit Monoclonal Antibody [JE64-48]

cat.: HA721060

Product Type:	Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity:	Human, Mouse, Rat
Applications:	WB, IF-Cell, IHC-P
Clonality:	Monoclonal
Clone number:	JE64-48

Form:	Liquid
Storage condition:	Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer:	1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration:	1ug/ul
Purification:	Protein A affinity purified.
Molecular weight:	Predicted band size: 108 kDa
Isotype:	IgG
Immunogen:	Synthetic peptide within human USO1 aa 1-50/962.
Positive control:	Hela cell lysate, 293T cell lysate, HepG2 cell lysate, A431 cell lysate, NIH/3T3 cell lysates, PC-12 cell lysates, rat epididymis tissue, human testis tissue, mouse cerebellum tissue, SH-SY5Y.
Subcellular location:	Cytosol, Golgi apparatus membrane.
Recommended Dilutions: WB IF-Cell IHC-P	1:1,000 1:400 1:100
Uniprot #:	SwissProt: O60763 Human \| Q9Z1Z0 Mouse \| P41542 Rat
Alternative names:	General vesicular transport factor General vesicular transport factor p115 P115 Protein USO1 homolog TAP Transcytosis associated protein Transcytosis-associated protein Uso1 USO1_HUMAN VDP Vesicle docking protein Vesicle docking protein p115 Vesicle-docking protein

Images

Fig1: Western blot analysis of USO1 on different lysates with Rabbit anti-USO1 antibody (HA721060) at 1/1,000 dilution.

Lane 1: Hela cell lysate
Lane 2: 293T cell lysate
Lane 3: HepG2 cell lysate
Lane 4: A431 cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 108 kDa
Observed band size: 108 kDa

Exposure time: 1 minute;

6% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721060) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

Fig2: Western blot analysis of USO1 on NIH/3T3 cell lysates with Rabbit anti-USO1 antibody (HA721060) at 1/1,000 dilution.

Lysates/proteins at 10 µg/Lane.

Predicted band size: 108 kDa
Observed band size: 108 kDa

Exposure time: 1 minute;

6% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721060) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

	Fig3: Western blot analysis of USO1 on PC-12 cell lysates with Rabbit anti-USO1 antibody (HA721060) at 1/1,000 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 108 kDa Observed band size: 108 kDa Exposure time: 1 minute; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721060) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
	Fig4: Immunohistochemical analysis of paraffin-embedded rat epididymis tissue with Rabbit anti-USO1 antibody (HA721060) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA721060) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig5: Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-USO1 antibody (HA721060) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA721060) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Fig6: Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-USO1 antibody (HA721060) at 1/100 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA721060) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Fig7: Immunocytochemistry analysis of SH-SY5Y cells labeling USO1 with Rabbit anti-USO1 antibody (HA721060) at 1/50 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-USO1 antibody (HA721060) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 647, HA1127) were used as the secondary antibody at 1/1,000 dilution.

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.