Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse, Rat |
Applications: | WB, IHC-P |
Clonality: | Monoclonal |
Clone number: | JE65-47 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 59 kDa |
Isotype: | IgG |
Immunogen: | Recombinant protein within human ATE1 aa 1-100/518. |
Positive control: | HL-60 cell lysates, mouse liver tissue lysate, mouse stomach tissue lysate, rat stomach tissue, human testis tissue, mouse liver tissue, rat liver tissue lysates. |
Subcellular location: | Cytoplasm, Nucleus. |
Recommended Dilutions:
WB IHC-P |
1:500-1:2,000 1:400 |
Uniprot #: | SwissProt: O95260 Human | Q9Z2A5 Mouse Entrez Gene: 293526 Rat |
Alternative names: | AI225793 Arginine tRNA protein transferase 1 Arginine-tRNA--protein transferase 1 Arginyl-tRNA--protein transferase 1 Arginyl-tRNA-protein transferase Arginyltransferase 1 Ate1 ATE1_HUMAN AW547406 EC 2.3.2.8 MGC26724 OTTHUMP00000020637 OTTHUMP00000058665 R transferase 1 R-transferase 1 |
Fig1:
Western blot analysis of ATE1 on HL-60 cell lysates with Rabbit anti-ATE1 antibody (HA721061) at 1/2,000 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 59 kDa Observed band size: 59 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721061) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of ATE1 on different lysates with Rabbit anti-ATE1 antibody (HA721061) at 1/500 dilution. Lane 1: Mouse liver tissue lysate Lane 2: Mouse stomach tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 59 kDa Observed band size: 59 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721061) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
Fig3:
Immunohistochemical analysis of paraffin-embedded rat stomach tissue with Rabbit anti-ATE1 antibody (HA721061) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721061) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-ATE1 antibody (HA721061) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721061) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-ATE1 antibody (HA721061) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721061) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Fig6:
Western blot analysis of ATE1 on rat liver tissue lysates with Rabbit anti-ATE1 antibody (HA721061) at 1/500 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 59 kDa Observed band size: 59 kDa Exposure time: 1 minute; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721061) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |