ATE1 Recombinant Rabbit Monoclonal Antibody [JE65-47]
cat.: HA721061
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P
Clonality: Monoclonal
Clone number: JE65-47
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 59 kDa
Isotype: IgG
Immunogen: Recombinant protein within human ATE1 aa 1-100/518.
Positive control: HL-60 cell lysates, mouse liver tissue lysate, mouse stomach tissue lysate, rat stomach tissue, human testis tissue, mouse liver tissue, rat liver tissue lysates.
Subcellular location: Cytoplasm, Nucleus.
Recommended Dilutions:
  WB
  IHC-P

1:500-1:2,000
1:400
Uniprot #: SwissProt: O95260 Human | Q9Z2A5 Mouse
Entrez Gene: 293526 Rat
Alternative names: AI225793 Arginine tRNA protein transferase 1 Arginine-tRNA--protein transferase 1 Arginyl-tRNA--protein transferase 1 Arginyl-tRNA-protein transferase Arginyltransferase 1 Ate1 ATE1_HUMAN AW547406 EC 2.3.2.8 MGC26724 OTTHUMP00000020637 OTTHUMP00000058665 R transferase 1 R-transferase 1
Images
HA721061_1.jpg Fig1: Western blot analysis of ATE1 on HL-60 cell lysates with Rabbit anti-ATE1 antibody (HA721061) at 1/2,000 dilution.

Lysates/proteins at 10 µg/Lane.

Predicted band size: 59 kDa
Observed band size: 59 kDa

Exposure time: 2 minutes;

12% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721061) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
HA721061_2.jpg Fig2: Western blot analysis of ATE1 on different lysates with Rabbit anti-ATE1 antibody (HA721061) at 1/500 dilution.

Lane 1: Mouse liver tissue lysate
Lane 2: Mouse stomach tissue lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 59 kDa
Observed band size: 59 kDa

Exposure time: 2 minutes;

8% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721061) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
HA721061_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded rat stomach tissue with Rabbit anti-ATE1 antibody (HA721061) at 1/400 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721061) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721061_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-ATE1 antibody (HA721061) at 1/400 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721061) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721061_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-ATE1 antibody (HA721061) at 1/400 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721061) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721061_6.jpg Fig6: Western blot analysis of ATE1 on rat liver tissue lysates with Rabbit anti-ATE1 antibody (HA721061) at 1/500 dilution.

Lysates/proteins at 20 µg/Lane.

Predicted band size: 59 kDa
Observed band size: 59 kDa

Exposure time: 1 minute;

12% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721061) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.