CRCP Recombinant Rabbit Monoclonal Antibody [JE65-42]
cat.: HA721062
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB, IF-Cell, IHC-P
Clonality: Monoclonal
Clone number: JE65-42
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 17 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human CRCP aa 10-60/148.
Positive control: MCF-7 cell lysates, human liver carcinoma tissue, mouse small intestine tissue, SH-SY5Y.
Subcellular location: Cell membrane, Nucleus.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
1:500
1:200
1:100
Uniprot #: SwissProt: O75575 Human | O35427 Mouse
Alternative names: Calcitonin gene related peptide receptor component Calcitonin gene related peptide receptor component protein Calcitonin gene-related peptide-receptor component protein CGRP receptor component protein CGRP-RCP CGRP-receptor component protein CGRPRCP CRCP DNA-directed RNA polymerase III subunit RPC9 HsC17 RCP RCP9 RNA polymerase III subunit C9 RPC9_HUMAN
Images
HA721062_1.jpg Fig1: Western blot analysis of CRCP on MCF-7 cell lysates with Rabbit anti-CRCP antibody (HA721062) at 1/500 dilution.

Lysates/proteins at 10 µg/Lane.

Predicted band size: 17 kDa
Observed band size: 17 kDa

Exposure time: 2 minutes;

15% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721062) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
HA721062_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue with Rabbit anti-CRCP antibody (HA721062) at 1/100 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721062) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721062_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue with Rabbit anti-CRCP antibody (HA721062) at 1/100 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721062) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721062_4.jpg Fig4: Immunocytochemistry analysis of SH-SY5Y cells labeling CRCP with Rabbit anti-CRCP antibody (HA721062) at 1/200 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-CRCP antibody (HA721062) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.