CRCP Recombinant Rabbit Monoclonal Antibody [JE65-42]
cat.: HA721062
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE65-42 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 17 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human CRCP aa 10-60/148. |
| Positive control: | MCF-7 cell lysates, human liver carcinoma tissue, mouse small intestine tissue, SH-SY5Y. |
| Subcellular location: | Cell membrane, Nucleus. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:500 1:200 1:100 |
| Uniprot #: | SwissProt: O75575 Human | O35427 Mouse |
| Alternative names: | Calcitonin gene related peptide receptor component Calcitonin gene related peptide receptor component protein Calcitonin gene-related peptide-receptor component protein CGRP receptor component protein CGRP-RCP CGRP-receptor component protein CGRPRCP CRCP DNA-directed RNA polymerase III subunit RPC9 HsC17 RCP RCP9 RNA polymerase III subunit C9 RPC9_HUMAN |
Images
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Fig1:
Western blot analysis of CRCP on MCF-7 cell lysates with Rabbit anti-CRCP antibody (HA721062) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 17 kDa Observed band size: 17 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721062) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue with Rabbit anti-CRCP antibody (HA721062) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721062) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue with Rabbit anti-CRCP antibody (HA721062) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721062) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunocytochemistry analysis of SH-SY5Y cells labeling CRCP with Rabbit anti-CRCP antibody (HA721062) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-CRCP antibody (HA721062) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.