Adiponectin Recombinant Rabbit Monoclonal Antibody [JE65-49]
cat.: HA721064
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE65-49 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 27 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within mouse Adiponectin aa 1-247/247. |
| Positive control: | Mouse colon tissue lysates, mouse heart tissue lysates, Mouse white adipose lysate, Mouse brown adipose tissue lysate, NCI-H441, mouse colon tissue. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:2,000 1:50 1:1,000 |
| Uniprot #: | SwissProt: Q60994 Mouse |
| Alternative names: | 30 kDa adipocyte complement related protein 30 kDa adipocyte complement-related protein ACDC Acrp 30 ACRP30 ADIPO_HUMAN Adipocyte Adipocyte C1q and collagen domain containing protein Adipocyte complement related 30 kDa protein Adipocyte complement related protein of 30 kDa Adipocyte complement-related 30 kDa protein adipocyte-specific secretory protein Adiponectin Adiponectin precursor adiponectin, C1Q and collagen domain containing Adipoq Adipose most abundant gene transcript 1 Adipose most abundant gene transcript 1 protein Adipose specific collagen like factor ADIPQTL1 ADPN APM 1 apM-1 APM1 C1q and collagen domain-containing protein GBP 28 GBP28 Gelatin binding protein Gelatin binding protein 28 gelatin-binding protein 28 Gelatin-binding protein OTTHUMP00000210047 |
Images
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Fig1:
Western blot analysis of Adiponectin on mouse colon tissue lysates with Rabbit anti-Adiponectin antibody (HA721064) at 1/500 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 27 kDa Observed band size: 34 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721064) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of Adiponectin on mouse heart tissue lysates with Rabbit anti-Adiponectin antibody (HA721064) at 1/500 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 27 kDa Observed band size: 34 kDa Exposure time: 1 minute; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721064) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Western blot analysis of Adiponectin on different lysates with Rabbit anti-Adiponectin antibody (HA721064) at 1/2,000 dilution. Lane 1: Mouse white adipose lysate Lane 2: Mouse brown adipose tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 27 kDa Observed band size: 34 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721064) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig4:
Immunocytochemistry analysis of NCI-H441 cells labeling Adiponectin with Rabbit anti-Adiponectin antibody (HA721064) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Adiponectin antibody (HA721064) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 647, HA1127) were used as the secondary antibody at 1/1,000 dilution. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-Adiponectin antibody (HA721064) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721064) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.