Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
---|---|
Species reactivity: | Human |
Applications: | WB |
Clonality: | Monoclonal |
Clone number: | JE65-50 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 71 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within human SLC25A12 aa 565-614/614. |
Positive control: | Daudi cell lysate, K562 cell lysate. |
Subcellular location: | Centriolar satellite, cilium basal body, nucleus, adherens junction. |
Recommended Dilutions:
WB |
1:500 |
Uniprot #: | SwissProt: Q9Y2D8 Human |
Alternative names: | ADIP ADIP_HUMAN Afadin and alpha actinin binding protein Afadin DIL domain interacting protein Afadin DIL domain-interacting protein Afadin- and alpha-actinin-binding protein FLJ10848 KIAA0923 MGC75026 OTTHUMP00000011574 SSX 2 interacting protein SSX2 interacting protein SSX2-interacting protein SSX2IP Synovial sarcoma X breakpoint 2 interacting protein " |
Fig1:
Western blot analysis of SSX2IP on different lysates with Rabbit anti-SSX2IP antibody (HA721065) at 1/500 dilution. Lane 1: Daudi cell lysate Lane 2: K562 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 71 kDa Observed band size: 80/90 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721065) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |