SLC25A12 Recombinant Rabbit Monoclonal Antibody [JE65-52]
cat.: HA721066
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE65-52 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 75 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human SLC25A12 aa 601-650/678. |
| Positive control: | Hela cell lysate, Raji cell lysate, RAW264.7 cell lysate, rat kidney tissue lysate, human kidney tissue, mouse heart tissue. |
| Subcellular location: | Mitochondrion inner membrane. |
| Recommended Dilutions:
WB IHC-P |
1:500 1:100 |
| Uniprot #: | SwissProt: O75746 Human | Q8BH59 Mouse Entrez Gene: 362145 Rat |
| Alternative names: | AGC1 Araceli hiperlarga ARALAR ARALAR1 Calcium binding mitochondrial carrier superfamily member Calcium binding mitochondrial carrier superfamily member Aralar1 Calcium-binding mitochondrial carrier protein Aralar1 CMC1_HUMAN Mitochondrial aspartate glutamate carrier 1 SLC25A12 Solute carrier family 25 (aspartate/glutamate carrier) member 12 Solute carrier family 25 (mitochondrial carrier Aralar) member 12 Solute carrier family 25 member 12 solute carrier family 25, member 12 |
Images
|
Fig1:
Western blot analysis of SLC25A12 on different lysates with Rabbit anti-SLC25A12 antibody (HA721066) at 1/500 dilution. Lane 1: Hela cell lysate Lane 2: Raji cell lysate Lane 3: RAW264.7 cell lysate Lane 4: Rat kidney tissue lysate (20 µg/Lane) Lysates/proteins at 10 µg/Lane. Predicted band size: 75 kDa Observed band size: 70 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721066) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-SLC25A12 antibody (HA721066) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721066) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-SLC25A12 antibody (HA721066) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721066) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.