TIM44 Recombinant Rabbit Monoclonal Antibody [JE65-55]
cat.: HA721067
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE65-55 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 51 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human TIM44 aa 1-280/452. |
| Positive control: | PC-3 cell lysate, HeLa cell lysate, K-562 cell lysate, HepG2 cell lysate, LNCaP cell lysate, PC-12 cell lysate. |
| Subcellular location: | Mitochondrion inner membrane, Mitochondrion matrix. |
| Recommended Dilutions:
WB |
1:5,000 |
| Uniprot #: | SwissProt: O43615 Human | O35094 Rat |
| Alternative names: | DKFZp686H05241 MIMT44 Mitochondrial inner membrane translocase TIM44 Translocase of inner mitochondrial membrane 44 Translocase of inner mitochondrial membrane 44 homolog (yeast) |
Images
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Fig1:
Western blot analysis of TIM44 on different lysates with Rabbit anti-TIM44 antibody (HA721067) at 1/5,000 dilution. Lane 1: PC-3 cell lysate Lane 2: HeLa cell lysate Lane 3: K-562 cell lysate Lane 4: HepG2 cell lysate Lane 5: LNCaP cell lysate Lane 6: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 51 kDa Observed band size: 45 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721067) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.