CSDE1 Recombinant Rabbit Monoclonal Antibody [JE65-67]
cat.: HA721071
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | JE65-67 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 89 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human CSDE1 aa 401-450/798. |
| Positive control: | HeLa cell lysate, A431 cell lysate, RAW264.7 cell lysate, PC-12 cell lysate, PC-12. |
| Subcellular location: | Cytoplasm, Stress granule, P-body. |
| Recommended Dilutions:
WB IF-Cell FC |
1:1,000 1:100 1:1,000 |
| Uniprot #: | SwissProt: O75534 Human | Q91W50 Mouse | P18395 Rat |
| Alternative names: | Cold shock domain containing E1 RNA binding Cold shock domain containing protein E1 Cold shock domain-containing protein E1 Csde1 CSDE1_HUMAN D1S155E DKFZp779B0247 DKFZp779J1455 FLJ26882 N-ras upstream gene protein NRAS related Nras upstream gene protein NRU Protein UNR RP5 1000E10.3 UNR UNR protein Upstream of NRAS |
Images
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Fig1:
Western blot analysis of CSDE1 on different lysates with Rabbit anti-CSDE1 antibody (HA721071) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: A431 cell lysate Lane 3: RAW264.7 cell lysate Lane 4: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 89 kDa Observed band size: 89 kDa Exposure time: Lane 1: 13 seconds; Lane 2-4: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721071) at 1/1,000 dilution was used in primary antibody dilution (K1803) at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunocytochemistry analysis of PC-12 cells labeling CSDE1 with Rabbit anti-CSDE1 antibody (HA721071) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CSDE1 antibody (HA721071) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Flow cytometric analysis of PC-12 cells labeling CSDE1. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721071, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.