CSDE1 Recombinant Rabbit Monoclonal Antibody [JE65-67]
cat.: HA721071
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE65-67 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 89 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human CSDE1 aa 401-450/798. |
| Positive control: | A431 cell lysate, RAW264.7 cell lysate, 293 cell lysate, Hela cell lysate, PC-12 cell lysates. |
| Subcellular location: | Cytoplasm, Stress granule, P-body. |
| Recommended Dilutions:
WB |
1:500-1:1,000 |
| Uniprot #: | SwissProt: O75534 Human | Q91W50 Mouse | P18395 Rat |
| Alternative names: | Cold shock domain containing E1 RNA binding Cold shock domain containing protein E1 Cold shock domain-containing protein E1 Csde1 CSDE1_HUMAN D1S155E DKFZp779B0247 DKFZp779J1455 FLJ26882 N-ras upstream gene protein NRAS related Nras upstream gene protein NRU Protein UNR RP5 1000E10.3 UNR UNR protein Upstream of NRAS |
Images
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Fig1:
Western blot analysis of CSDE1 on different lysates with Rabbit anti-CSDE1 antibody (HA721071) at 1/1,000 dilution. Lane 1: A431 cell lysate Lane 2: RAW264.7 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 89 kDa Observed band size: 89 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721071) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of CSDE1 on different lysates with Rabbit anti-CSDE1 antibody (HA721071) at 1/500 dilution. Lane 1: 293 cell lysate Lane 2: Hela cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 89 kDa Observed band size: 89 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721071) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Western blot analysis of CSDE1 on PC-12 cell lysates with Rabbit anti-CSDE1 antibody (HA721071) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 89 kDa Observed band size: 89 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721071) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.