ELOVL5 Recombinant Rabbit Monoclonal Antibody [JE65-70]
cat.: HA721072
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE65-70 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 35 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human ELOVL5 aa 250-299/299. |
| Positive control: | Mouse brain tissue lysates, rat lung tissue lysates. |
| Subcellular location: | Endoplasmic reticulum membrane, dendrite. |
| Recommended Dilutions:
WB |
1:1,000-1:5,000 |
| Uniprot #: | SwissProt: Q9NYP7 Human | Q8BHI7 Mouse | Q920L7 Rat |
| Alternative names: | 3 keto acyl CoA synthase ELOVL5 3-keto acyl-CoA synthase Elovl5 dJ483K16.1 Elongation of very long chain fatty acids like 5 Elongation of very long chain fatty acids protein 5 ELOV5_HUMAN ELOVL 5 ELOVL family member 5 ELOVL family member 5 elongation of long chain fatty acids ELOVL family member 5, elongation of long chain fatty acids (FEN1/Elo2, SUR4/Elo3-like, yeast) ELOVL fatty acid elongase 5 ELOVL2 elovl5 Fatty acid elongase 1 hELO1 Homolog of yeast long chain polyunsaturated fatty acid elongation enzyme 2 OTTHUMP00000017867 RP3 483K16.1 RP3-483K16.1 |
Images
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Fig1:
Western blot analysis of ELOVL5 on mouse brain tissue lysates with Rabbit anti-ELOVL5 antibody (HA721072) at 1/5,000 dilution. Lysates/proteins at 40 µg/Lane. Predicted band size: 35 kDa Observed band size: 33 kDa Exposure time: 46 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721072) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of ELOVL5 on rat lung tissue lysates with Rabbit anti-ELOVL5 antibody (HA721072) at 1/1,000 dilution. Lysates/proteins at 40 µg/Lane. Predicted band size: 35 kDa Observed band size: 33 kDa Exposure time: 4 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721072) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.