Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human, Rat |
Applications: | WB |
Clonality: | Monoclonal |
Clone number: | JE65-70 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 35 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within human ELOVL5 aa 250-299/299. |
Positive control: | Rat kidney tissue lysate, rat brain tissue lysate. |
Subcellular location: | Endoplasmic reticulum membrane, dendrite. |
Recommended Dilutions:
WB |
1:500 |
Uniprot #: | SwissProt: Q9NYP7 Human | Q920L7 Rat |
Alternative names: | 3 keto acyl CoA synthase ELOVL5 3-keto acyl-CoA synthase Elovl5 dJ483K16.1 Elongation of very long chain fatty acids like 5 Elongation of very long chain fatty acids protein 5 ELOV5_HUMAN ELOVL 5 ELOVL family member 5 ELOVL family member 5 elongation of long chain fatty acids ELOVL family member 5, elongation of long chain fatty acids (FEN1/Elo2, SUR4/Elo3-like, yeast) ELOVL fatty acid elongase 5 ELOVL2 elovl5 Fatty acid elongase 1 hELO1 Homolog of yeast long chain polyunsaturated fatty acid elongation enzyme 2 OTTHUMP00000017867 RP3 483K16.1 RP3-483K16.1 |
Fig1:
Western blot analysis of ELOVL5 on different lysates with Rabbit anti-ELOVL5 antibody (HA721072) at 1/500 dilution. Lane 1: Rat kidney tissue lysate Lane 2: Rat brain tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 35 kDa Observed band size: 38 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721072) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |