ELOVL5 Recombinant Rabbit Monoclonal Antibody [JE65-70]
cat.: HA721072
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Rat
Applications: WB
Clonality: Monoclonal
Clone number: JE65-70
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 35 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human ELOVL5 aa 250-299/299.
Positive control: Rat kidney tissue lysate, rat brain tissue lysate.
Subcellular location: Endoplasmic reticulum membrane, dendrite.
Recommended Dilutions:
  WB

1:500
Uniprot #: SwissProt: Q9NYP7 Human | Q920L7 Rat
Alternative names: 3 keto acyl CoA synthase ELOVL5 3-keto acyl-CoA synthase Elovl5 dJ483K16.1 Elongation of very long chain fatty acids like 5 Elongation of very long chain fatty acids protein 5 ELOV5_HUMAN ELOVL 5 ELOVL family member 5 ELOVL family member 5 elongation of long chain fatty acids ELOVL family member 5, elongation of long chain fatty acids (FEN1/Elo2, SUR4/Elo3-like, yeast) ELOVL fatty acid elongase 5 ELOVL2 elovl5 Fatty acid elongase 1 hELO1 Homolog of yeast long chain polyunsaturated fatty acid elongation enzyme 2 OTTHUMP00000017867 RP3 483K16.1 RP3-483K16.1
Images
HA721072_1.jpg Fig1: Western blot analysis of ELOVL5 on different lysates with Rabbit anti-ELOVL5 antibody (HA721072) at 1/500 dilution.

Lane 1: Rat kidney tissue lysate
Lane 2: Rat brain tissue lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 35 kDa
Observed band size: 38 kDa

Exposure time: 2 minutes;

12% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721072) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.