OXGR1 Recombinant Rabbit Monoclonal Antibody [JE64-04]
cat.: HA721074
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE64-04 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 38 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human OXGR1 aa 171-220/337. |
| Positive control: | Hela cell lysate, rat brain tissue lysate, mouse testis tissue lysates, rat testis tissue lysates. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB |
1:500-1:2,000 |
| Uniprot #: | SwissProt: Q96P68 Human | Q6IYF8 Mouse | Q6Y1R5 Rat |
| Alternative names: | 2 oxoglutarate receptor 1 2-oxoglutarate receptor 1 aKGR Alpha ketoglutarate receptor 1 Alpha-ketoglutarate receptor 1 G Protein Coupled Receptor 80 G Protein Coupled Receptor 99 G-protein coupled receptor 80 G-protein coupled receptor 99 GPR80 GPR99 MGC119206 MGC119207 MGC119208 OXGR 1 Oxgr1 OXGR1_HUMAN Oxoglutarate (alpha ketoglutarate) receptor 1 Oxoglutarate receptor 1 P2RY15 P2Y like GPCR P2Y like nucleotide receptor P2Y purinoceptor 15 P2Y-like GPCR P2Y-like nucleotide receptor P2Y15 |
Images
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Fig1:
Western blot analysis of OXGR1 on different lysates with Rabbit anti-OXGR1 antibody (HA721074) at 1/500 dilution. Lane 1: Hela cell lysate (10 µg/Lane) Lane 2: Rat brain tissue lysate (20 µg/Lane) Predicted band size: 38 kDa Observed band size: 38 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721074) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of OXGR1 on mouse testis tissue lysates with Rabbit anti-OXGR1 antibody (HA721074) at 1/500 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 38 kDa Observed band size: 38 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721074) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Western blot analysis of OXGR1 on rat testis tissue lysates with Rabbit anti-OXGR1 antibody (HA721074) at 1/2,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 38 kDa Observed band size: 38 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721074) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.