NUP50 Recombinant Rabbit Monoclonal Antibody [JE64-06]
cat.: HA721075
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE64-06 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 50 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human NUP50 aa 11-110/468. |
| Positive control: | Jurkat cell lysate, mouse testis tissue lysate, rat large intestine tissue, human liver carcinoma tissue, human colon carcinoma tissue, mouse hippocampus tissue, mouse brain tissue. |
| Subcellular location: | Nuclear pore complex, Nucleus membrane. |
| Recommended Dilutions:
WB IHC-P |
1:500 1:100-1:400 |
| Uniprot #: | SwissProt: Q9UKX7 Human | Q9JIH2 Mouse | O08587 Rat |
| Alternative names: | 50 kDa nucleoporin NPAP60 NPAP60L Nuclear pore associated protein 60L Nuclear pore complex protein Nup50 Nuclear pore-associated protein 60 kDa-like Nucleoporin 50 Nucleoporin 50kDa Nucleoporin Nup50 Nup50 NUP50_HUMAN PRO1146 |
Images
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Fig1:
Western blot analysis of NUP50 on different lysates with Rabbit anti-NUP50 antibody (HA721075) at 1/2,000 dilution. Lane 1: Jurkat cell lysate Lane 2: Mouse testis tissue lysate Lysates/proteins at 30 µg/Lane. Exposure time: 65 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA721075, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 50 kDa Observed band size: 50 kDa |
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Fig2:
Immunohistochemical analysis of paraffin-embedded rat large intestine tissue with Rabbit anti-NUP50 antibody (HA721075) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721075) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue with Rabbit anti-NUP50 antibody (HA721075) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721075) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-NUP50 antibody (HA721075) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721075) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-NUP50 antibody (HA721075) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721075) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-NUP50 antibody (HA721075) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721075) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.