GRSF1 Recombinant Rabbit Monoclonal Antibody [JE65-34]
cat.: HA721076
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, IF-Cell
Clonality: Monoclonal
Clone number: JE65-34
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 53 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human GRSF1 aa 350-400/480.
Positive control: Hela cell lysate, 293 cell lysate, Jurkat cell lysate, HepG2 cell lysate, Hela.
Subcellular location: Mitochondrion matrix; Cytoplasm.
Recommended Dilutions:
  WB
  IF-Cell

1:500
1:50
Uniprot #: SwissProt: Q12849 Human
Alternative names: G rich RNA sequence binding factor 1 G rich sequence binding factor 1 G-rich sequence factor 1 GRSF-1 GRSF1 GRSF1_HUMAN
Images
HA721076_1.jpg Fig1: Western blot analysis of GRSF1 on different lysates with Rabbit anti-GRSF1 antibody (HA721076) at 1/500 dilution.

Lane 1: Hela cell lysate
Lane 2: 293 cell lysate
Lane 3: Jurkat cell lysate
Lane 4: HepG2 cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 53 kDa
Observed band size: 53 kDa

Exposure time: 2 minutes;

10% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721076) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
HA721076_2.jpg Fig2: Immunocytochemistry analysis of Hela cells labeling GRSF1 with Rabbit anti-GRSF1 antibody (HA721076) at 1/50 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-GRSF1 antibody (HA721076) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) were used as the secondary antibody at 1/1,000 dilution.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.