GRSF1 Recombinant Rabbit Monoclonal Antibody [JE65-34]
cat.: HA721076
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | JE65-34 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 53 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human GRSF1 aa 350-400/480. |
| Positive control: | Hela cell lysate, 293 cell lysate, Jurkat cell lysate, HepG2 cell lysate, Hela. |
| Subcellular location: | Mitochondrion matrix; Cytoplasm. |
| Recommended Dilutions:
WB IF-Cell |
1:500 1:50 |
| Uniprot #: | SwissProt: Q12849 Human |
| Alternative names: | G rich RNA sequence binding factor 1 G rich sequence binding factor 1 G-rich sequence factor 1 GRSF-1 GRSF1 GRSF1_HUMAN |
Images
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Fig1:
Immunocytochemistry analysis of Hela cells labeling GRSF1 with Rabbit anti-GRSF1 antibody (HA721076) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-GRSF1 antibody (HA721076) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) were used as the secondary antibody at 1/1,000 dilution. |
|
Fig2:
Western blot analysis of GRSF1 on different lysates with Rabbit anti-GRSF1 antibody (HA721076) at 1/2,000 dilution. Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate Lane 2: HepG2 (Human liver cancer cell) cell lysate Lane 3: 293T (Human embryonic kidney cell) cell lysate Lane 4: Jurkat (Human T-lymphoblastic cells) cell lysate Lysates/proteins at 10 µg/Lane. Exposure time: 3 minutes; ECL: K1801 Negative expression of GRSF1 protein in Daudi cell is consistent with the predicted expression pattern. Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA721076, 1/2,000 in primary antibody dilution buffer 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 53 kDa Observed band size: 53 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.