UCHL5IP Recombinant Rabbit Monoclonal Antibody [JE65-32]
cat.: HA721077
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE65-32 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 40 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human UCHL5IP aa 150-300/368. |
| Positive control: | HeLa cell lysate, 293T cell lysate, Jurkat cell lysate, human kidney tissue. |
| Subcellular location: | Centrosome, spindle. |
| Recommended Dilutions:
WB IHC-P |
1:5,000 1:400 |
| Uniprot #: | SwissProt: Q99871 Human |
| Alternative names: | 26S proteasome-associated UCH interacting protein 1 26S proteasome-associated UCH37-interacting protein 1 Expressed-Xq28STS protein HAUS augmin-like complex subunit 7 Haus7 HAUS7_HUMAN HSXQ28ORF UCH37 interacting protein 1 UCHL5 interacting protein UCHL5-interacting protein UCHL5IP UIP1 X-linked protein STS1769 |
Images
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Fig1:
Western blot analysis of UCHL5IP on different lysates with Rabbit anti-UCHL5IP antibody (HA721077) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: 293T cell lysate Lane 3: Jurkat cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 40 kDa Observed band size: 40 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721077) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-UCHL5IP antibody (HA721077) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721077) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.